弹性蛋白酶制作囊性动脉瘤模型的实验研究

Reproduction of experimental saccular aneurysm by corrosion of arterial wall with elastase

目的 建立病理形态与自然动脉瘤更接近、更适于实验研究的动物模型。方法 以弹性蛋白酶破坏SD大鼠 (n =10 )右颈外动脉起始部 ,在距其 1mm处结扎并剪断 ,建立直径为 1mm的囊性动脉瘤模型 ,测量不同时间动脉瘤的大小。喂养 4周后灌注固定取动脉瘤标本 ,通过HE和VanGieson染色方法对瘤壁进行病理评价。结果 动脉瘤模型的病理形态与颅内自然动脉瘤极为相似 ,内膜不完整 ,弹力层及肌层断裂或消失 ,代之以增生的胶原纤维组织。4周时动脉瘤大小较制作时稍有增大 (P <0 0 1)。结论 本实验动脉瘤模型的病理形态及发展特性与颅内自然动脉瘤相似 ,为动脉瘤的实验研究提供了较理想的动物模型。

Objecive To reproduce an experimental model of aneurysm more which is simulating intracranial aneurysm pathomorphologically to serve further research on aneurysm. Methods Saccular aneurysms of the carotid artery were reproduced by corrosion of initial segment of the external carotid artery (1mm long) with elastase in 10 SD rats. After 4 weeks, the size of the aneurysms was measured. Then the aneurysms were fixed by intra-arterial perfusion of formalin and were resected. The pathological characteristics were evaluated with hematoxylin-eosin and Van Gieson staining. Results The pathological features of the experimental aneurysms showed that the tunica intima was interrupted, the elastic and muscular layers were disrupted or disappeared, replaced by proliferative collagen fiber, simulating natural aneurysms. After 4 weeks, the size of aneurysms was larger compared with that in the beginning (P<0.01). Conclusions This experimental aneurysm was similar to natural aneurysm patho-morphologically and provided an ideal model for experimental studies on the aneurysm.