二磷酸盐对关节磨屑刺激单核细胞分泌IL-1β的抑制作用

Inhibitory effect of bisphosphonates on IL-1β expression of human monocytes induced by wear debris

目的:研究二磷酸盐对关节磨屑刺激单核细胞分泌IL-β的影响。方法:分离培养人外周血单核细胞,加入关节磨屑及不同浓度的阿伦膦酸钠,ELISA检测细胞上清中IL-1β的含量,原位杂交检测IL-1β mRNA的表达。结果:关节磨屑组IL-1β及基因的表达显著高于对照组(P<0.01),不同浓度的阿伦膦酸钠组IL-1β及基因的表达明显低于关节磨屑组(P<0.01)。结论:阿伦膦酸钠能通过下调人外周血单核细胞IL-1β3及mRNA的表达,对人工关节松动可能起防治作用。

Objective:To study the effect of bisphosphonates on IL-1β expression of human monocytes with wear particles stimulation. Methods: In vitro human peripheral blood mononuclear cell (PBMC) were cultured with wear particles and bisphosphonates of different concentration, then productions of IL-1β and IL-1β mRNA were determined by ELISA and in situ hybridization techniques. Results:The production of IL-1β were increased significantly when wear particles was added,compared with control group (P<0.01). And bisphosphonates reduced the level of IL-1β. Hybridization demonstrated bisphosphonates might down regulate IL-1β mRNA expression. Conclusion:Bisphosphonates can inhibit IL-1β expression induced by wear debris and may be used in the prevention and treatment of aseptic loosening of prosthesis.