脲酶的制备及终点法检测尿素氮试剂盒的配制

Preparation of Urease and Prescription of Reagent for Determination of Urea by a Method of Ending

报道了以刀豆为原料制备脲酶的一种简便方法,其总收率为37.1％,比活达43.6μmol·min^(-1)/mg蛋白.用该酶配制的尿素氮试剂盒检测误差仅为1.5％,在4℃可保存三个月.EDTA和甘油是脲酶的有效稳定剂.

The present paper covers a simple method for preparing urease from jack bean. In terms of the method, the total yield was 37. 1% and the specific activity was 43. 6 μmol ?min-1/ mg protein. The error-detecting of the reagent for the determination of urea made up from the urease is only 1. 5% and the storable time is 3 months at 4 ℃. EDTA and glycerin are the stabilizers of the reagent.