救心复脉注射液对豚鼠心室肌细胞L型钙电流的影响

Effect of Jiuxinfumai injection on L-type calcium currents in single guinea pig ventricular myocytes

目的 :探讨救心复脉注射液 (枳实提取液 )有效成分辛弗林 (311)、N 甲基酪胺 (4 17)对豚鼠心室肌细胞膜L型钙通道电流 (ICa L)的影响。方法 :用酶解法分离单个心室肌细胞 ,采用全细胞膜片钳技术 ,观察辛弗林、N 甲基酪胺对豚鼠心室肌细胞膜ICa L的影响。结果 :用辛弗林 (10 ,2 5 ,5 0 ,10 0mmol/L)时能增大ICa L,增加率分别为 8.2 7% ,2 7.2 9% ,4 1.0 1%和 4 8.74 % (P <0 .0 5 )。用N 甲基酪胺 (10 ,2 5 ,5 0 ,10 0mmol/L)时能增大ICa L,增加率分别为 10 .0 5 % ,30 .12 % ,4 3.0 5 %和 5 1.90 % (P <0 .0 5 )。辛弗林和N 甲基酪胺只改变电流幅度 ,不改变I V曲线形状。结论 :辛弗林和N 甲基酪胺有浓度依赖性的增大豚鼠心室肌细胞ICa L。

Objective To determine the effect of 311 and 417, both active ingredients isolated from Jiuxinfumai injection (Citrus Aurantium) on L-type calcium currents (I Ca-L)in ventricular myocytes of guinea pigs.Methods Single myocytes were dissociated by enzymatic dissociation method. The whole-cell patch-clamp recording technique was used to record the change of calcium current after the administration of 311and 417. Results 311(10,25,50,100 mmol/L)increased the I Ca-L by 8.27%, 27.29%, 41.01%,and 48.74% (P<0.05), respectively. 417(10,25,50,100 mmol/L) increased the I Ca-L by 10.05%, 30.12%,43.05%,and 51.90% (P<0.05), respectively. Both 311 and 417 changed the I Ca-L significantly in a concentration-dependent manner. They did not change the shape of I-V cruves.Conclusion 311 and 417 can increase I Ca-L in ventricular myocytes of guinea pigs in a dose-response manner.