高血压对大鼠血管内皮细胞PPAR-gamma表达水平的影响

Increased expression of peroxisome proliferator-activated receptor-gamma in endothelial cells of spontaneously hypertensive rats

目的 :探讨高血压对大鼠血管内皮细胞 (ECs )PPAR gamma蛋白质和mRNA表达水平的影响。方法 :应用免疫组织化学法结合图像信号分析技术 ,以WKY大鼠为正常对照 ,检测 4周 ,16周自发性高血压大鼠 (SHR)ECs核内PPAR gamma蛋白质表达水平 ,原代培养ECs并传代 (≤ 3代 ) ,应用蛋白质免疫印迹和RT PCR技术 ,检测ECsPPAR gamma蛋白质和mRNA表达水平。结果 :4周SHR血压较同龄WKY大鼠轻度升高 (P <0 .0 5 ) ,其ECsPPAR gamma的蛋白质和mRNA水平略高于同龄WKY大鼠 ,但差异均无统计学意义 (P >0 .0 5 )。 16周SHR血压与 16周WKY大鼠相比明显增高 (P <0 .0 1) ,16周SHRECsPPAR gamma的蛋白质和mRNA水平约为 16周WKY的 1.5倍 (P <0 .0 1) ,且 16周SHRPPAR gamma蛋白质和mRNA水平约为 4周SHR的 2 .5倍 (P <0 .0 1) ,而对照组 16周WKY大鼠PPAR gamma水平较 4周WKY仅升高不到 1倍 (P <0 .0 1)。结论 :随SHR血压的升高和高血压病程的延长 ,ECsPPAR

Objective To evaluate the relationship between hypertension and the expression level of PPAR-gamma in endothelial cells (ECs) of spontaneously hypertensive rats (SHR). Methods PPAR-gamma protein expression in ECs of 4-week and 16-week SHR was detected by immunohistochemistral technique and analyzed by image acquiring and analysis system, and age matched Wistar-Kyoto rats (WKY) were used as controls.The aortic ECs of different-aged rats were primarily cultured, and the protein and mRNA level of PPAR-gamma in cultured ECs (≤3 passages) were detected by Western blotting and reverse transcription-polymerase chain reaction (RT-PCR).Results The blood pressure of 4-week SHR was slightly higher than those of 4-week WKY (P<0.05). The protein and mRNA levels of PPAR-gamma in 4-week SHR were also higher than that of 4-week WKY, but the differences had no statistical significance (P>0.05). Compared with the 16-week WKY, the blood pressure of 16-week SHR greatly increased (P<0.01), and the protein and mRNA levels of PPAR-gamma in ECs from 16w SHR were approximately 1.5 times higher than those of the age matched WKY (P<0.01). The protein and mRNA levels of PPAR-gamma in ECs from 16-week SHR were 2.5 times higher than those of 4-week SHR (P<0.01), while in 16-week WKY it was only 1.5 times higher than those of 4-week WKY (P<0.01). Conclusion In accordance with the hypertension progress, the protein and mRNA levels of PPAR-gamma in SHR can significantly increase.