长期口服复方尼尔雌醇和小剂量17β-雌二醇对去卵巢大鼠海马结构内神经生长因子表达的影响

Effects of long-term replacement therapy of compound nylestriol tablet or low-dose 17 beta-estradiol on the expression of nerve growth factor in OVX rat hippocampal formation

目的 :观察长期雌激素缺乏大鼠海马结构内神经生长因子 (NGF)表达的变化 ,同时比较长期口服复方尼尔雌醇和小剂量 17β 雌二醇对去卵巢大鼠海马结构内NGF表达的作用。方法 :7月龄SD大鼠按体重随机分成 5组 :正常对照组、假手术组 (SHAM )、去卵巢组 (OVX)、17β 雌二醇预防干预组 (OVX/ERT)和复方尼尔雌醇预防干预组 (OVX/NL)。 5组均在去卵巢后 35周处死。用免疫组织化学的方法 (SABC法 )显示老年大鼠海马结构内NGF的表达 ,细胞计数及图像分析法观察去卵巢大鼠海马结构内NGF表达的变化。结果 :正常组、SHAM组、OVX/ERT组、OVX/NL组海马结构内的各亚区均可见较多NGF阳性神经元。OVX组海马结构内各亚区NGF阳性神经元较少。OVX组海马结构内各亚区NGF阳性神经元数量和平均光密度均低于其它 4组 (P <0 .0 5 )。结论 :长期雌激素缺乏将导致大鼠海马结构内NGF表达的下降 ,长期口服小剂量 17β 雌二醇和复方尼尔雌醇补充治疗有利于去卵巢大鼠海马结构内神经元NGF的正常表达 ,从而发挥雌激素的神经营养作用。复方尼尔雌醇获得了与小剂量 17β

Objective To determine the effects of long-term estrogen deficiency and replacement therapy of compound nylestriol tablet or low-dose 17 beta-estradiol on the expression of nerve growth factor (NGF) in rat hippocampal formation.Methods Fifty 7 month-old female Sprague-Dawley rats were randomly divided into 5 groups: normal control, sham operated(SHAM), ovariectomized(OVX), OVX plus 17 beta-estradiol (OVX/ERT), and OVX plus compound nylestriol tablet (OVX/NL)groups. Immunohistochemistry of NGF was used to quantitatively determine the levels of expression of NGF using cell counting and imaging system in ovariectomized rat hippocampal formation.Results The number and optical density of NGF-positive neurons of all hippocampal subregions and dentate gyrus in OVX rats were obviously lower than those of the normal control, SHAM ,OVX/NL, and OVX/ERT rats.Conclusion Long-term estrogen deficiency can lead to a decrease of NGF expression in hippocampal formation,while the replacement of low-dose 17 beta-estradiol or compound nylestriol tablet can equally preserve the expression of NGF to a normal level, showing a neurotrophic effect of estrogen.