人直肠癌组织中肿瘤浸润淋巴细胞的形态学观察

Morphological Observation of Tumor Infiltrating Lymphocytes in Tissue of Human Rectal Cancer

目的 :探讨人胃癌组织中肿瘤浸润淋巴细胞 (TIL )的形态学特征。方法 :采用免疫组织化学 ,光镜和电镜方法观察人直肠癌组织中肿瘤细胞浸润淋巴细胞的分布和形态学变化。结果 :肿瘤浸润淋巴细胞主要分布在癌周区 ,在病变早期 ,肿瘤浸润淋巴细胞数量较多 ,分布集中 ;在病变晚期 ,淋巴细胞数量较少 ,分布均匀。病变早期 TIL数量明显高于病变晚期 (P<0 .0 1)。电镜下 ,TIL大体可分为两种形态 :一种具有一般淋巴细胞的典型构造 ,核大 ,胞浆少 ,细胞器也较少 ;另一种 TIL核较圆 ,胞浆较多 ,内有丰富内质网、溶酶体、线粒体及颗粒。 TIL与肿瘤浸润树突状细胞 (TIDC)有接触。结论 :本试验提示直肠癌组织中 TIL数量多少与肿瘤的进展有关。 TIL与 TIDC关系密切。

Objective:To Study the morphological characteristics of tumor infiltrating lymphocytes (TIL) in tissue of human rectal cancer.Methods:The distribution and morphological changes of TIL were observed by light microscopy, electron microscopy and immunohisochemisitry.Results:TIL mainly appeared in the tumor-surrounding tissue.There was many more TILs which were very concentrated in the earlier stage, but the amount reduced and the distribution became evenly in the later stage. The number of TIL was much more in the earlier stage than in the later stage (P<0.01). Under electron microscope, there were two types of morphology in TIL. One has typical structure of ordinary lymphocyte such as big nucleus ,a little cytoplasm and fewer cell organells . The other had a round nucleus and fewer Cytoplasm. There were plentiful endoplasmic reticulm, lysosome, mitochondria and granule in Cytoplasm. There existed the contacts between TIL and tumor infiltrating dendritic cell (TIDC).Conclusions:The number of TIL were related to the progress of tumor. There was a close relationship between TIL and TIDC.