依那普利对自发性高血压大鼠心脏局部血管紧张素转换酶及左室肥厚的作用

Effect of enalapril on angiotensin converting enzyme and left ventricular hypert rophy in the spontaneous hypertensive rats

目的 研究自发性高血压大鼠 (SHR)心脏局部血管紧张素转换酶 (ACE)活性对左室肥厚的影响及依那普利的作用。方法 选用 1 2w龄SHR 40只 ,随机分为两组 ,一组给予依那普利 30mg/ (kg·d)治疗 ,另一组作为对照 ,同时设一同周龄的WKY作为正常对照 ,用病理学图像分析法检测SHR左心室壁厚度、心肌细胞体积比例 (CVF)和心肌血管周围胶原面积和管腔面积比例 (PVCA) ,放免法测定血管紧张素Ⅱ (AngⅡ )浓度 ,荧光法测定ACE活性。结果 (1 )SHR治疗组 (SHR T)BW/LV与正常对照组 (WKY C)相比无差异 (P >0 0 5) ;(2 )SHR C组AngⅡ浓度为 (5 780± 3 734)ng/mg组织 ,显著高于WKY C组〔(2 72 3± 0 84)ng/mg组织〕(P <0 0 5) ;而依那普利治疗后SHR T组AngⅡ浓度为 (2 757± 2 71 7)ng/mg组织 ,与WKY C组相比无显著差异。 (3)SHR T、SHR C、WKY C三组ACE活性分别为 (0 47± 0 1 2 )单位 / (ml·mg组织 ) ,(0 60± 0 1 3)单位 / (ml·mg组织 ) ,(0 45± 0 1 9)单位 / (ml·mg组织 ) ,SHR T组与SHR C组比较有显著差异 (P <0 0 1 ) ,SHR T组与WKY C组比较无显著差异 (P >0 0 5)。 (4)SHR T组CVF和PVCA分别为 (1 98± 1 57) %和 (0 68± 0 1 9) % ,显著低于SHR C组〔(5 1 1± 2 2 5) % ,(1 2 0±

Objective To investigate the influence o f angiotensin converting enzyme (ACE) activity and enalapril on the left ventricul ar hypertrophy (LVH) in the spontaneous hypertensive rats(SHR).Meth ods 40 12-week- old SHRs were divided randomly into enalapril 3 0 mg/(kg·d) treatment group and control group, at the same time, WKY with the match age was normal control. The thickness of the wall of left ventricular, the BM/LV, CVF and PVCA were measured by pathological examination and morphological analysis. The concentration of AngⅡ was measured with RIA. The activity of ACE was determined wit fluorescence analysis. Results (1) BM / LV in SHR-T was similar to that of WKY-C (P>0.05). (2)The concentrati on of Ang Ⅱin heart of SHR-C (5.780±3.734 ng/mg tissue) was much higher than that of WKY-C (2.723±0.84 ng/mg tissue). But after the treatment of enalapril, there was no significant difference of AngⅡconcentration between SHR-T (2.75 7±2.717 ng/mg tissue) and WKY-C. (3)The activity of ACE in SHR-T, SHR-C and WKY-C were 0.47±0.12 units/〔ml·mg(tissue)〕, 0.60±0.13 units/〔ml·mg(tissu e)〕, 0.45±0.19 units/〔ml·mg(tissue)〕, respectively. The activity of ACE in heart of SHR-C was higher than the other groups (P<0.01), and there is no d ifference between SHR-T and WKY-C (P>05). (4) CVF and PVCA in heart of S HR-T were 1.98%±1.57% and 0.68%±0.19%, respectively, which were lower than th ose of SHR-C 〔(5.11±2.25)% and (1.20±0.19)%, P<0.05)and similar to those of WKY-C (1.70%± 0.63% and 0.5%±70.17%, P>0.05). Co nclusions ACEI can inhibit the activity of local ACE, decrease the concentration of AngⅡ and reversed LVH.