磷霉素与替考拉宁对90株革兰阳性球菌的联合药敏研究

Antibacterial Activity of Fosfomycin Combined with Teicoplanin Against Gram-positive Cocci

目的 评价磷霉素与替考拉宁对 90株革兰阳性 ( G+)球菌的体外联合抗菌效应。方法 采用棋盘法设计 ,微量肉汤稀释法测定 ;测定不同浓度组合的抗菌药物对 90株临床分离的革兰阳性球菌的联合药敏 ,计算 FIC指数并判定联合效应 ;FIC≤ 0 .5为协同作用 ,0 .5 <FIC≤ 1为相加作用 ,1<FIC≤ 2为无关作用 ,FIC>2为拮抗作用。结果 磷霉素与替考拉宁联合应用后 ,其 MIC50 显著降低 ;FIC指数分布 :FIC≤ 0 .5占 6 0 .0 0 %～ 93.30 % ;0 .5 <FIC≤ 1占 6 .70 %～ 4 0 .0 0 % ;1<FIC≤ 2为 0 ;FIC>2为 0。结论 磷霉素与替考拉宁联合用药后 ,对革兰阳性球菌基本表现为协同作用和相加作用 ,并以协同作用为主 ,无关作用较少 。

OBJECTIVE To evaluate the antibiotic effects of fosfomycin combined with teicoplanin against 90 strains of Grams positive cocci. METHODS The protocol was designed by checkboard method and the MICs of fosfomycin alone and combined with teicoplanin against the 90 strains of Gram positive cocci were determined by broth dilution method, the FIC index was calculated according to MIC results. RESULTS The MIC 50 of fosfomycin were reduced significantly when combined with vancomycin. The percentage of the FIC index less than 0.5, from 0.5 to 1, from 1 to 2, more than 2 was 60.00-93.30%, 6.70-40.00%, 0, and 0, respectively. CONCLUSIONS The results suggested that synergism and additivity of fosfomycin combined with teicoplanin against 90 strains of Gram positive cocci be the main actions, there are the little autonomy and no antagonism.