磁性颗粒固相分离剂的制备及其在放射免疫分析中的应用

Preparation and application of magnetic particle solid-phase second antibody for RIA

本文研制了一种用于放射免疫测试的磁颗粒固相二抗分离试剂。将粗羊抗兔IgG二抗溶液纯化,采用氨基硅烷化修饰的磁性颗粒选择不同的酸度经戊二醛活化后,在不同的酸度下与纯化的二抗交联。同时进行了未活化磁性颗粒对二抗的吸附实验。放射免疫药盒测试结果为:未活化磁性颗粒固相二抗结合率低且极不稳定;活化后磁性颗粒固相二抗结合率高且稳定性好,并提高了放免药盒的灵敏度,标准曲线的相关性系数达0.9950。在液相分离法与磁性颗粒固相分离法的放射免疫测试比较中,其测得样品浓度的相关性也达到了0.9895。因此该磁性颗粒固相二抗是一个可用于放射免疫测试的通用分离试剂。

A separation reagent of magnetic particle solid-phase second antibody for RIA has been studied. Amine silane modulated magnetic particles were activated using glutaraldehyde and coupled with purified second antibody at different pH values. And the experiment on adsorption of purified second antibody by non-activated magnetic particles at pH 8 was carried out simultaneously. The RIA test demonstrated that the binding percentage of non-activated magnetic particle solid-phase was lower and its stability was less than that of the activated magnetic particle solid-phase . The sensitivity was increased and the correlation coefficient of standard cure was 0.9950 for the activated magnetic particle solid-phase second antibody for CEA RIA. The correlation coefficient of the testing concertration in RIA between separations with liquid-phase and solid-phase was 0.9895. Therefore, it is possible for the magnetic particle solid-phase to be used as a general separation reagent for RIA.