重度妊娠高血压综合征患者血清诱导的脐血管内皮细胞凋亡失衡与肿瘤坏死因子的关系

Apoptosis imbalance induced by sera from patients of severe pregnancy induced hypertension in umbilical vein endothelial cells in vitro is associated with tumor necrosis factor

目的:探讨重度妊娠高血压综合征(简称妊高征)患者血清对体外培养的脐血管内皮细胞(HUVEC)凋亡和对抑／促凋亡基因Bcl-2／Bax表达的影响以及与肿瘤坏死因子α(TNFα)的关系。方法:体外培养HIJVEC,加入重度妊高征患者血清和TNFα作为处理因素,免疫细胞化学检测Bcl-2／Bax蛋白表达;流式细胞仪做凋亡细胞记数;扫描电镜观察细胞变化。结果:轻重度妊高征患者血清处理后的HUVEC Bcl-2／Bax表达失衡,凋亡细胞数增加,扫描电镜可见经TNFα处理后的内皮细胞变圆,微绒毛消失,密度增高,可见凋亡小体。结论:TNFα可诱导体外培养的脐血管内皮细胞凋亡,并可引起凋亡相关基因Bcl-2／Bax表达失衡,可能是引起妊高征血管内皮细胞损伤的原因之一。

Objective: To investigate the apoptosis induced by sera from patients of severe pregnancy induced hypertension (PIH) in umbilical vein endothelial cells (HUVEC) in vitro. The effect of sera from PIH patients on the expression of the antiapoptotic/pro-apoptotic protein Bcl-2/Bax and whether this effect is associated with tumor necrosis factorα(TNFα) is also studied. Methods: HUVEC (ECV304)are pretreated with sera from patients of severe PIH in vitro, Bcl-2/Bax protein expression are examined by immunocytochemistry. Apoptotic cell counting carried out by flowcytometry is to test the number of apoptotic cells. Scan electron microscopy is used to observe the features of the cells. Results: TNFαand sera from severe PIH patients increase the number of apoptotic cells. The balance of Bcl-2/Bax protein is disrupted when HUVEC is pretreated with sera from patients of severe PIH, apoptotic body is observed when HUVEC is pretreated with TNFα. Conclusion: TNFα could induce HUVEC apoptosis, which lead to the imbalance of anti-apoptotic/pro-apoptotic protein Bcl-2/Bax. It suggest that the apoptotic imbalance induced by TNFαmay be one factor in damage of endothelial cells in PIH.