石菖蒲挥发油中β-细辛醚在小鼠体内的药代动力学

Pharmacokinetics of β-Asarone in the volatile oil from Acorus. Tatarinowii Schott in mice

目的 :研究石菖蒲挥发油中 β 细辛醚在小鼠体内的药物代谢动力学。 方法 :采用气相色谱法测定 β 细辛醚的血药浓度 ,色谱柱 :HP 35毛细管气相色谱柱 (内径 :0 .2 5mm ;长 :30m ;涂膜厚 :0 .2 5 μm) ;程序升温 :起始温度为 30℃ ,6℃ /min升至 2 2 0℃ ,保持 5min ;进样口温度 ;2 5 0℃ ;检测器温度 :32 0℃ ;进样量 :2 .0 μL;无分流进样 ;溶剂 :甲醇 ;载气 :氦气 ;柱头压 :5 .5× 1 0 4帕斯卡 ;内标物 :a 萘酚。结果与结论 :建立了用气相色谱法测定β 细辛醚血药浓度的方法 ,β 细辛醚与内标物的峰面积之比 (Y)与浓度 (C)之间的回归方程为 :Y =0 .0 1 889C +1 .75 5× 1 0 -3 (n =6 ,r =0 .9999)。线性范围 :0 .0 4 μg·mL-1 - 4 0 .0 μg·mL-1 。小鼠全血中最低检测浓度为 :0 .0 4 μg/mL。β 细辛醚在全血中的回收率为 :95 .3%,RSD为 3.92 %。小鼠口服石菖蒲挥发油后 ,β 细辛醚在体内的药时过程为线性动力学过程 ,符合一级吸收二室模型 ,t1 /2α为6 .5min ,t1 /2 ( β) 为 93.6min。

AIM: To study the pharmacokinetics of β Asarone in the volatile oil from Acorus. Tatarinowii Schott. in mice. METHODS: We developed a GC method for β Asarone determination in blood. The GC system consisted of HP 35 capillary column with Helium as carrier gas, oven starting temperature set at 30℃ , then was raised to 220℃ at a rate of 6℃/min , stayed for 5 minutes, injector temperature set at 250℃ , detector temperature at 320℃ . Injection volume was 2.0 μL with splitless inject mode. Methanol was used as solvent and α naphthol as interior standard substance. CONCLUSION: The calibration curve in blood was linear in the range of 0.04μg/ml to 40μg/ml . The minimum detection limit was 0.04μg/ml . The recovery for β Asarone in blood was 95.3% with RSD 3.92% . After oral administration of volatile oil from Acorus. Tatarinowii Schott., the intracorporal process of β Asarone in mice accorded with 2 compartment model with 1st order absorption. 6.5min and 93.6min were obtained as t 1/2α and t 1/2β respectively.