HPLC测定荆肤止痒颗粒中5-O-甲基维斯阿米醇苷的含量

Determination of 4′-O-β-D-glycosyl-5-O-methylvisamminol in Jingfuzhiyang Granules by RP HPLC

目的 :建立RP HPLC法测定荆肤止痒颗粒 (荆芥 ,地肤子 ,防风 ,山楂等 )中 5 O 甲基维斯阿米醇苷含量的方法。方法 :采用TurnerKromasilC1 8(4.6mm× 2 0 0mm ,5 μm)色谱柱 ;流动相为甲醇 - 0 .0 5mol·L-1 KH2 PO4溶液 醋酸 异丙醇(85∶1 70∶4∶4 ) ,检测波长为 2 5 4nm ,流速为 1 .0mL·min-1 ,柱温为 30℃。结果 :5 O 甲基维斯阿米醇苷在上述色谱条件下 ,分离效果好 ,在 0 .0 6 0 6～ 0 .6 0 6 μg范围内 ,线性关系良好 (r =0 .9998) ,加样回收率为 92 .2 %。 结论 :本方法较简单 ,准确 ,可作为产品的质量控制方法。

AIM: To establish a RP HPLC method for the determination of 4′ O β D glycosyl 5 O methylvisamminol in Jingfuzhiyang Granules (Herba Schizonepetae, Fructus kochiae, Radix Saposhnikoviae, Fructus Crataegi, etc.). METHODS: The sample was separated on Turnner Kromasil C 18 column (4.6mm×200mm,5μm) with mobile phase of methanol 0.05mol·L -1 potassium dihydrogen phosphate acetic acid isopropyl alcohol (85∶170∶4∶4,v/v). The detection wavelength was set at 254nm and the flow rate was 1.0mL·min -1 . The column temperature was 30℃. RESULTS: The calibration curve was linear in the range of 0.0606～0.606μg (r=0.9994). The average recovery was 92.2%. CONCLUSION: This method proves to be relatively simple and accurate, and available for the quality control of Jingfuzhiyang Granules