RP-HPLC法测定野菊花中蒙花苷含量

Determination of content of linarin in Flos Chrysanthemi Indici by RP-HPLC

目的建立野菊花中蒙花苷的含量测定方法。方法采用反相高效液相色谱法 ,DiamonsilTMC18柱 (2 5 0mm× 4 6mm ,5 μm) ,流动相为甲醇 水 磷酸 (体积比 5 7∶4 3∶0 0 4 ) ,检测波长为32 6nm。结果蒙花苷线性范围 0 4 8～ 9 70mg·L-1,r =0 9999(n =7) ;平均回收率为 98 4 % ,RSD为 2 0 % (n =9)。

Objective To establish a method for the determination of the content of linarin in Flos Chrysanthemi Indici by RP-HPLC.Methods The chromatographic condition included a DiamonsilTM C_(18) column ((250 mm)×(4.6 mm),(5 μm)) and the mobile phase consisting of methanol-water-phosphoric acid (V∶V∶(V=)57∶43∶0.04) and the absorbance was monitored at (326 nm).Results The calibration curve was linear over the range of 0.48～(9.70 mg·L^(-1)) for linarin (r=(0.999 9)).The average recovery was 98.4%(n=9)and the RSD was 2.0%.Conclusion The method can be used for the quality control of Flos Chrysanthemi Indici.