摘要
底物分子的引入会导致表面活性剂PSE水溶液CMC值增大.表面活性剂与超声处理配合使用可以有效地增强底物的溶解.在表面活性剂 底物 水体系中,高强度、长时间的超声处理对底物的溶解不利.超声波(强度为6.2W/cm2,时间为10min)与表面活性剂PSE(质量分数为0.015%)的配合使用可以降低表面活性剂用量,在增强甾体底物溶解性的同时,能够更好地维持转化阶段菌体酶的活性,有效地实现底物转化.
The presence of substrate leads to an increase in critical micelle concentration (CMC) of surfactant PSE compared with the normal CMC of PSE in aqueous solution. The solubility of substrate can′t be enhanced effectively in surfactant-substrate-water system when it is treated by ultrasonic irradiation with long time and high intensity. Using surfactant and ultrasonic irradiation jointly may enhance the substrate solubility effectively. Ultrasonic irradiation (6.24 W/cm^2, 10 min) and surfactant PSE (0.015%) used jointly could decrease the amount of surfactant needed, maintain the activity of enzyme and therefore realize the effective conversion of steroid substrate.
出处
《天津大学学报（自然科学与工程技术版）》
EI
CAS
CSCD
北大核心
2004年第11期941-944,共4页
Journal of Tianjin University：Science and Technology
基金
国家自然科学青年基金资助项目(29606008)
天津市自然科学基金资助项目(993607111).
关键词
甾体化合物
微生物转化
表面活性剂
超声波
溶解
steroid
microbial conversion
surfactant
ultrasonic irradiation
solubility
