阿尔茨海默病与血管性痴呆患者脑脊液tau蛋白及β-淀粉样蛋白_(1-42)浓度的对照研究

The comparison of cerebrospinal fluid levels of tau protein and β-amyloid peptide1-42 in the patients with Alzheimer's disease and those with vascular dementia

目的探讨脑脊液(CSF)中tau蛋白及β-淀粉样蛋白1-42(Aβ1-42)的浓度对阿尔茨海默病(AD)的诊断价值,寻找AD理想的生物学标志。方法用酶联免疫吸附法分别检测22例AD、20例血管性痴呆(VD)患者和21名正常对照者(对照组)CSF中tau蛋白及Aβ1-42水平。结果 (1)CSF中tau蛋白平均浓度:AD组(318±249)ng／L,VD组(219±190)ng／L,对照组(119±65)ng／L,AD组CSF中tau蛋白浓度明显高于对照组,差异有显著性意义(P<0.05);CSF中Aβ1-42越平均浓度:AD组(344±166)ng／L,VD组(467±223)ng／L,对照组(480±217)ng／L,AD组明显低于对照组,差异有显著性意义(P<0.05)。依据CSF中tau蛋白和Aβ1-42浓度对三组进行判别分析时,有44％的研究对象被正确判别。结论 CSF中tau蛋白浓度的升高和Aβ1-42浓度的降低,可能对AD的临床诊断有潜在的应用价值。

Objective To investigate the cerebrospinal fluid (CSF) levels of tau protein and β-amyloid peptide1-42( Aβ1-42 ) in the patients with Alzheimer's disease (AD) and their diagnostic value for AD. Methods The subjects were 22 patients with AD and 41 controls, including 20 patients with vascular dementia (VD) and 21 normal controls (NC). The CSF levels of tau protein and Aβ1-42 were measured with enzyme-linked immunosorbent assay. Results The mean CSF levels of tau were (318±249) ng/L in AD patients, (219±190) ng/L in VD, and (119±65) ng/L in NC, respectively, and the difference between AD and NC was statistically significant (P <0. 05). The mean CSF levels of Aβ1-42 were (344±166) ng/L in AD, (467±223) ng/L in VD, and (480±217) ng/L in NC, and the difference between AD and NC was statistically significant ( P < 0. 05 ). In discriminant analysis of combining the CSF levels of tau and Aβ1-42, only 44% of subjects were correctly classified. Conclusions Both the elevated CSF levels of tau protein and the decreased CSF levels of Aβ1-42 may be biomarkers for AD patients, which have potential utility in clinical diagnosing AD.