贡嘎蝠蛾幼虫肠道菌群的分析

Analysis on the HepialusGonggaensis' IntestinalBacterialFlora

贡嘎蝠蛾(Hepialusgonggaensis)幼虫是名贵中药材冬虫夏草(Cordycepssinensis)的优势寄主。研究贡嘎蝠蛾幼虫肠道菌群,对研究蝠蛾的营养生理,提高幼虫成活率,进而人工培殖冬虫夏草具有重要意义。实验以四川康定采集的贡嘎蝠蛾为实验材料,对贡嘎蝠蛾幼虫的肠道细菌进行了分离和鉴定。实验结果表明,贡嘎蝠蛾肠道细菌都属低温菌,最适生长温度15℃,在15℃培养2d后才长出典型菌落。从肠道中分离纯化出12个不同菌群,其中优势菌群为葡萄球菌,菌群数量为2.05×109mL-1,检出率100%,应为幼虫肠道中的常住菌群,其它菌群检出率均低于50%,可能为肠道中的过路菌群。生化鉴定大多数菌群的符合值都较低,这与蝠蛾生活于高海拔高寒地区,其肠道微生物与常见微生物菌群有较大差异一致。

The larvae of Hepialus gonggaensis are predominant host of Cordyceps sinensis, which can produce one kind of famous and expensive herb medicine in China and Asia area. It is important to determinate the intestinal microbial flora of larva for studying the nutritional physiology and improving the artificial breeding and increasing the larvae livability, which is in turn to benefit the artificial or half artificial cultivation of Cordyceps sinensis. In the experiment, intestinal microbial flora of H. gonggaensislarvae, collected from Kangding,Sichuan Province, have been isolated and identified. The results show that the intestinal microbial flora of H. gonggaensisbelongs to low-temperature bacteria. The bacteriagrew very slowly and the typical bacterial colony appeared on cultural plate only after 2 daysincubatedunder the most profitable temperature,15 ℃。Twelve different isolates of bacteria are isolated from the larve intestine by 5 sorts of cultural media. It shows that the predominant bacterium is Staphylococcus, whose viable count is 2.05×10^(9) mL^(-1), and the detection rate is 100%. It indicates that they should be the normal flora in the larva's intestine. The other strains can be isolated occutionath, they are supposed to be the passer microbes. The biochemical identity shows that the identifying values are relative low for most bacteria, which are consistent with the larvae's abnormal living environment.