Ri质粒介导西洋参高效转化体系的建立

072-03Establishment of efficient transformation system mediated by Ri plamid in Panax quinquefolium L.

由Ri质粒介导对西洋参叶片进行遗传转化,获得西洋参发根,并且对诱导发根的影响因素进行研究,建立了西洋参高效转化体系,为利用生物技术生产和提高西洋参中所含中药成分的研究奠定基础.

Genetic transformation was carried out into the leaves of Panax quinquefolium L. via Ri plasmid and their hairy roots were acquired. The factors influencing the inducement of hairy roots were also studied and efficient transformation system mediated by Ri plamid in P.quinquefolium L. was established, which provided the basis of production and promotion of the ingredients of the P.quinquefolium L..