一种新型肽核酸基因传感器阵列检测系统的构建

Construction of A New-style Detection System of Peptide Nucleic Acid Gene Sensors Array

目的 构建针对乙型肝炎病毒 (HBV)的肽核酸 (PNA)压电基因传感器 (PGS)阵列检测系统。方法 设计针对 HBV的 bis- PNA探针 ,将其固定在 PGS阵列表面 ,具体摸索了 bis- PNA探针与 HBV基因组 DNA杂交时的最佳 p H值、最佳离子浓度、最佳探针固定量 ,并结合自激式振荡电路 ,PESA V2 .0频率记录分析软件构建出PNA- PGS阵列检测系统。结果 杂交缓冲液 p H值为 6 .8、离子浓度为 2 0 mm ol/ L、探针浓度为 1.5 μm ol/ L 时杂交条件最为适宜。结论 成功地构建出了 HBV PNA- PGS阵列检测系统 ,为临床标本中 HBV的直接检测奠定了良好的实验基础。

OBJECTIVETo construct the detection system of HBV peptide nucleic acid (PNA) piezoelectric gene sensors (PGS) array. METHODSBis-PNA probe for the HBV was designed and immobilized on the surface of PGS array. And the optimal pH value, ion concentration, immobilization probe concentration were all definitely explored and determined when bis-PNA was hybridized with HBV genomic DNA. So the detection system was hence constructed by combining with self-oscillating circuit and PESA V2.0 software. RESULTSIt was most suitable for hybridization on the condition of pH value 6.8, 20 mmol/L ion concentration, 1.5 μmol/L probe concentration. CONCLUSIONS The HBV PNA-PGS detection system is successfully constructed, and hence solid experimental foundation is established for the direct detection of the clinical samples.