166例真菌感染分离鉴定及药敏试验结果分析

Significance of Fungus Identification and Drug Sensitive Test: Analysis of 166 Fungal Infection Cases

目的 分析 16 6例真菌感染阳性标本分离鉴定及药敏试验结果 ,以探讨其预防措施。方法 沙保弱培养基培养标本 ,用 ID真菌鉴定板条进行鉴定分型 ,纸片扩散法进行药敏实验。结果 16 6株真菌中 ,白色念珠菌感染率最高 ,占 74 .1% ,并且分离出了烟曲霉菌、构巢曲霉菌等少见菌株 ;分离的菌株对制霉菌素、两性霉素 B和伊曲康唑的敏感率较高 ,分别为 10 0 %、97.8%和 96 % ;对 5 -氟胞嘧啶和咪康唑的耐药率较高 ,分别为 7.3%和 8.6 %。结论 对于提高真菌的检测手段、及时进行真菌的培养和药敏实验、合理使用抗菌药物、减少真菌感染、特别是耐药菌株感染的发生有着重要的意义。

OBJECTIVETo analyze the results of identification and drug sensitive test of 166 fungal infection samples, so as to find measures for their prevention. METHODS Sabourand′s agar culture medium was used to culture fungi, ID identification strip was employed to identify the fungi and drug sensitive test was performed by disk diffusion test. RESULTSThe incidence of Candida albicans infection was the highest (74.1%). Also some rare fungal infections, such as Aspergillus fumigatus and A. nidulans, were confirmed. The separated fungi were relatively sensitive to nystatin (NYS, 100%), amphotericin B (AMB, 97.8%), itraconazole (ITRA, 96%), and resistant to 5-fluorocytosine (5-FC, 7.3%) and miconazole(MIC, 8.6%). CONCLUSIONSDetection technique of fungal infection should be improved and anti-fungal medicine should be used reasonably according to the result of drug sensitive test so that the incidence of fungal infection, especially fungi-resistant infection could be reduced.