荧光原位杂交法对血培养标本中细菌和真菌鉴定效果评价

Rapid Identification of Bacteria and Fungus in Positive Blood Culture by Fluorescent in situ Hybridization

目的 建立一种阳性血培养标本的检测和鉴定的快速方法。方法 采用荧光原位杂交法 ,Bact/Alert仪器报警后 ,取阳性血培养液 15 μl于玻片上 ,全细胞杂交 1h ,同时将阳性标本转种培养基平板 ,用VITEK仪器法鉴定 ,比较杂交法与仪器法的符合率。结果 杂交法与仪器法测定了 118份临床阳性标本 ,葡萄球菌属的符合率96 3% ,链球菌属和肠球菌属的符合率 10 0 % ,革兰阴性杆菌的符合率 86 8% ,白色念珠菌的符合率 10 0 % ,总符合率 91 5 %。结论 联合应用Bact/Alert血培养仪及荧光原位杂交法 ,能显著缩短阳性血培养标本中的细菌和真菌的鉴定时间。

OBJECTIVE To develop a rapid method for identification of bacteria and fungus in positive blood culture. METHODS Once Bact/Alert gave a flag, withdrawed the 15μl blood onto slide, and hybridized 1 hour, and the positive blood was spiked on media plates, then identified with VITEK standard method. RESULTS Total 118 positive samples were detected,the coherence rate of Staphylococcus spp. was 96.3%,that of Streptococcus spp. and Enterococcus spp. was 100%, the coherence of G- bacillus was 86.8%, that of Candida albicans was 100%,crude agreement rate was 91.5%. CONCLUSIONS Combination of Bact/Alert and fluorescent in situ hybridization, could reduce the identification time obviously for bacteria and fungus in positive blood culture. It is worthwhile to be used for hybridization method on blood culture in routine work.