腹腔镜手术器械灭菌的方法和监测

Monitoring in Sterilization of Laparoscope

目的 腹腔镜手术器械的灭菌及其灭菌效果的监测。方法 灭菌方法采用酶化洁液浸泡及高压水枪对腹腔镜手术器械进行清洗后浸泡于 2 %的戊二醛中 10h ;监测方法采用枯草芽胞杆菌黑色变种芽胞菌片检测戊二醛溶液、甲醛熏箱的灭菌效果。结果 所作监测均为无菌生长。结论 腹腔镜器械灭菌的前提是必须彻底清洗 ,对灭菌措施进行监测更为重要。

OBJECTIVE To study the effect of a new method on the sterilization of laparoscope. METHODS Way of sterilization: after soaking with enzymatic detergent and cleaning by high pressure water, the laparoscope was soaked in 2% glutaraldehyde for 10 hours. Way of monitoring: The Bacillus subtilis was applied to observe the sterilization effect of the glutaraldehyde solution and the Formalin. RESULTS All the detection showed no bacterial growth. CONCLUSIONS For laparoscope sterilization, it should be cleaned thoroughly in advance. Furthermore, it is more important to monitor the ways of sterilization.