^(103)Pd粒子联合加热41℃对人肝癌细胞7402细胞毒作用的实验研究

The Cytotoxic Effect of ^(103)Pd Particle Radiotherapy Combinded with 41℃ Heating on Human Hepatocarcinoma Cell Line Bel-7402

目的 研究10 3 Pd粒子与加热 41℃对人肝癌细胞株 (Bel -740 2 )的细胞毒作用 ,了解10 3 Pd粒子对热疗后残存的肝癌细胞的细胞毒作用。方法 以体外培养的人肝癌细胞 -740 2为研究对象 ,采用对照分组、水浴加温法、体外细胞毒试验 (MTT)、凋亡实验 (Hoechst3 3 2 5 8荧光染色法 )的方法以及方差分析 ,观察10 3 Pd粒子放疗联合加热 41℃对Bel -740 2细胞的生长抑制作用。结果 10 3Pd粒子对 41℃加热后的人肝癌细胞 -740 2有明显的细胞抑制作用 ,强于单纯10 3 Pd粒子放疗。结论 人肝癌细胞 -740 2对10 3 Pd粒子放疗敏感 ,10 3 Pd粒子联合加热 41℃有增效作用。提示 :10 3 Pd粒子对热疗后残存的肝癌细胞有细胞毒作用。

Objective To investigate the cytotoxic effect of 103 palladium( 103 Pd) particle radiotherapy combined with 41℃ heating on human hepatocarcinoma cell line Bel-7402. Methods Human hepatocarcinoma cells (Bel-7402) were treated by water-heating and/or 103 Pd particle. The cell growth was determined with MTT assay, and the cell apoptosis was detected by the fluorescent stain of Hoechst33258. Results The cytotoxic effect of 103 Pd radiotherapy combinded with 41℃ heating on Bel-7402 cells was stronger than that of only 103 Pd radiotherapy. Conclusion 103 Pd radiotherapy and 41℃ heating had a synergistic effect on hepatocarcinoma cell Bel-7402, and 103 Pd radiotherapy had the cytotoxic effect on the surviving Bel-7402 cells after hot therapy.