蒺藜中一个新的有机酸成分的分离与鉴定

A new organic acid from Tribulus terrestris

目的 :研究蒺藜的水溶性成分。方法 :采用 80 %乙醇提取、硅胶柱层析 (用不同比例二氯甲烷∶甲醇混合溶剂洗脱 )分离和光谱分析鉴定化合物结构。结果和结论 :从蒺藜中分离出 1种微量水溶性成分 ,经 UV、IR、1 HNMR、1 3CNMR、DEPT、2 D- NMR、MS等光谱分析鉴定为新的有机酸 ,分子式为 C4 H6 N4 O3,相对分子质量为 15 8,命名为蒺藜酸 (terrestric acid,6 -氨基- 4 -羰基 - 1,2 ,3,4 -四氢 - 1,3,5 -三唑 - 2 -甲酸 )。

Objective:To investigate the water soluble chemical constituent from Tribulus terrestris L..Methods:The raw material was extracted by 80% ethanol and the compound was isolated by means of chromatography on silica gel column.The structure of compound was elucidated by spectral analysis (UV,IR, 1HNMR, 13 CNMR,DEPT,2D-NMR,MS).Results and Conclusion:A water soluble constituent has been isolated from Tribulus terrestris L..Based on the spectroscopic data,it is identified as a new organic acid named terrestric acid (6-amino-4-oxo-1,2,3,4-tetrahydro-1,3,5-triazine-2-carboxylic acid).