空间分辨荧光分析技术

Space resolved Fluorescence Techniques

空间分辨荧光分析技术突破了传统荧光分析的局限 ,为获得空间定位信息提供了技术保障。系统地综述了构成该技术的共焦荧光法、全内反射荧光法、多光子荧光法以及近场荧光法等 4种方法的原理、特点、发展及其应用 ,并且强调了其在单分子测定中的作用。引用文献 6 4篇。

Space-resolved fluorescence techniques provide the opportunity to gain the space-locating information of samples. Four fluorescence approaches with space-resolved capability, i.e. confocal fluorescence multi-photon excitation, total internal reflection fluorescence and near-field fluorescence, are reviewed with 64 references. Their applications in single-molecule detection are emphasized.