大鼠深低温停循环模型的建立

Establishment of the rat model of deep hypothermic circulatory arrest

目的 建立大鼠深低温停循环复苏模型。方法 采用体外插管法建立大鼠闭胸式深低温停循环复苏模型,并在深低温停循环时,用冷脑保护液间断给予脑组织灌流。结果 用大鼠建立深低温停循环复苏模型:呼吸的自主恢复率为80％,心跳恢复率为100％。结论 大鼠深低温停循环复苏模型的建立,对研究脑组织低温状态下的病理生理学变化和临床应用低温进行脑保护提供了基本条件。

Objective To establish the animal model of deep hypothermic circulatory arrest (DHCA) in Sprague-Dawley rats. Methods The animal model of closed-chest DHCA was established in rats by means of external cannulation. The brain was given with low-flow discontinuous perfusion with cold cerebral protective fluid. The physiological signs were measured. Results The gradual perfusion is more fit to the physiological blood circuit and beneficial for preserving the stability of arterial blood gas. The recovery rates of respiration were 80% and those of the heart beat were 100%. Conclusion A feasible rat model of DHCA in which neurologic changes can be demonstrated will offer a convenience for the study of cerebral pathophysiologic changes in the deep hypothermic conditions and clinical application of deep hypothermia for protecting brain.