胃饲乙醇预处理增强肝脏对缺血再灌注损伤耐受性的实验研究

Experimental study on the reinforement of ischemia-reperfusion toler ance of the liver by ethanol preconditioning in rat

目的 探讨胃饲乙醇预处理能否延长大鼠肝脏耐受入肝血流阻断的安全时限。方法 Wistar大鼠随机分组 ,动物乙醇预处理后制作缺血再灌注模型 ,于手术后 0h～ 7d内不同时相活杀大鼠 ,留取血及肝脏标本保存待检。结果 入肝血流阻断 12 0min时 ,胃饲乙醇预处理组 14d存活率为10 0 % ,缺血组存活率为 70 % ,差异显著 ;预处理组和缺血组较正常组ALT值明显升高 ,72h后基本恢复 ,缺血末期和再灌注 6h ,预处理组和缺血组两者间差异显著 ;预处理组和缺血组ATP值均低于正常组 ,预处理组再灌注 12h后恢复 ,缺血组再灌注 72h恢复正常 ;病理观察见预处理组较缺血组表现为较轻的病理损害 ;免疫组化结果 :正常肝脏仅见弱阳性表达 ,散在分布于库普弗细胞。预处理组和缺血组表现为肝细胞阳性表达 ,术后随再灌注时相的延长 ,HO 1表达逐渐增强 ,预处理组表达明显强于缺血组 ,相差显著。结论 胃饲乙醇预处理能够增强肝脏对缺血再灌注损伤的耐受性 ,大鼠肝脏耐受入肝血流阻断的安全时限可延长至 12

Objective To investigate the maximum tolerance limi t of rats to hepatic inflow occlusion by ethanol gavage preconditioning. Methods Adult Wistar rats were randomly divided into different groups. The animal model of ischemia-reperfusion was established after alcohol were given by gastric gavage to experimental group. Rats were killed at different time points between 0 h to 7 d after reperfusion. Serum and liver tissue sample were kept. The animal survival rate was calculated following 14 d of reperfusion after hepatic inflow occlusion of 120 min. The animal survival rate was 100 percent in EPC group and 70 percent in I/R group. Results There was obvious difference between two groups (P<0.05). The serum ALT in EPC group was lower than that in I/R group (P<0.05); ATP content in liver tissue were higher in EPC group than that in I/R group (P<0.05). Histological findings showed less injury in EPC group. Immunochemistry analysis showed that HO-1(+) was expressed in Kupffer cells in normal liver at a low level. HO-1(+) was found in hepatocytes plasms at higher level in EPC group than in I/R group, and there was significant difference between two groups (P<0.05). Conclusions The 120 min is the maximum limit of rats to hepatic inflow occlusion by ethanol gavage preconditioning. HO-1 may play an important role in this effect.