高渗盐水处理对失血性休克兔红细胞免疫黏附功能及抗细菌感染的影响

Effects of hypertonic saline on erythrocyte adherence function and bacterial infection of hemorrhagic shock rabbits

目的 :探讨高渗盐水处理 ,对失血性休克兔红细胞免疫黏附功能和对大肠杆菌攻击抵抗力的影响。方法 :将 6 0只兔分为6组 ,每组 10只 ,第 1组为假休克组 :仅在局麻下插管加肝素 ;第 2、3组经颈动脉放血导致兔休克。 30min后 ,第 1、2组兔分别给予生理盐水及含 1× 10 9/kg大肠杆菌的平衡盐液 ;第 2组兔给予 75g/L盐水及含 1× 10 9/kg大肠杆菌的平衡盐液 ,然后观察 3组动物的存活率。另取兔 30只 ,分组和失血性休克模型的制作同上 ,但处理所用的平衡盐液中不含大肠杆菌。以C3b受体花环及IC花环形成实验 ,检测休克后 5h兔红细胞的免疫黏附功能。结果 :高渗盐水处理组兔红细胞C3b受体花环的形成率显著高于生理盐水治疗组 (P <0 .0 1) ;而IC花环形成率则显著低于生理盐水处理组 (P <0 .0 1)。另外 ,高渗盐水处理能显著提高失血性休克兔对大肠杆菌攻击的抵抗力。结论

AIM: To explore the effect of hypertonic saline on the erythrocyte adherence function and bacterial infection of hemorrhagic shock rabbits. METHODS: 60 Japanese rabbits were randomly divided into 6 groups, 10 for each group. Artery catheterization and heparin were given to the rabbits in group 1 (sham shock group). Hemorrhagic shock model was set up by bleeding resulting from carotid artery catheter in group 2 (normal saline group )and group 3 (hypertonic saline group). 30 minutes after shock, the rabbits in group 1 and group 2 were treated with normal saline and balanced salt solution containing 1×10~9/kg E.coli, respectively. And the rabbits in group 3 were treated with 75 g/L NaCl solution and balanced salt solution containing 1×10~9/kg E.coli. Then the survival rates of the rabbits in group 1-3 were observed. Rabbits in group 4-6 were same treatment as received, group 1-3, respectively, except that there was no E.coli in balanced salt solution. The erythrocyte immune adherence function of rabbits in group 4-6 were detected 5 hours after shock by RBC-C3bR and RBC-IC rosette forming assays. RESULTS: The survival rate of rabbits in hypertonic saline group was significantly higher than that in normal saline group.The RBC-C3bR rosette forming rate of the normal saline treated rabbits were pronouncedly decreased, while RBC-IC rosette forming rate was notably elevated, as compared with those of either sham shock group or hypertonic saline group(P<0.01). Hypertonic saline markedly increased RBC C3bR rosette forming rate. CONCLUSION: The above findings suggest that hypertonic salt solution can remarkably improve the depressed erythrocyte immune adherence function and enhance the rabbit’s resistance to (E.coli) challenge after hemorrhagic shock.