二烯丙基二硫阻滞人胃癌细胞周期的作用及机制

Role and Mechanisms of Diallyl Disulfide to Arrest The Cell Cycle Progression of MGC803 Gastric Cancer Cell

目的观察二烯丙基二硫(DADS)对人胃癌MGC803细胞周期的影响及其机制。方法应用流式细胞术分析人胃癌MGC803细胞在DADS处理前后细胞周期分布的变化。采用免疫组化链霉素抗生物素蛋白过氧化酶方法(SP)检测DADS处理前后p21WAF1、Rb、p53、p21ras、Cmyc基因的表达。结果20、25、30、35μ·ml-1DADS的不同浓度处理的MGC803细胞G1期细胞所占比例无影响,S期细胞数减少,而G2期细胞则明显增加,与未处理组比较有显著性差异(P<0.05)。DADS作用后的MGC803细胞,p21WAF1表达明显增加,而Rb表达呈弱阳性,p53、p21ras、Cmyc表达阴性。DMSO处理的MGC803细胞亦呈现同样改变。结论DADS可以阻滞MGC803细胞于G2/M期。其作用机制可能与上调p21WAF1、Rb基因表达,下调p53、ras、Cmyc基因表达有关。

Objective To explore the effects and mechanisms of diallyl disulfide (DADS) on the cell cycle of human gastric cancer MGC803 cells.Methods Cell cycle of MGC803 cell treated with DADS in vitro was analyzed by flow cytometry. The expression of p21 WAF1 ,Rb,p53,p21 ras and C myc proteins was examined by streptavidin peroxidase conjugated method (SP).Results MGC803 cells were treated with DADS at the concenstation of 20, 25, 30, 35μg/ml, respectively. The results showed that cell percentage in S phase of the DADS treated MGC803 cells was decreased, whereas cell percentage in G 2 phase was significantly increased( P < 0.05 ), and little change in G 1 phase was found. At same time the markedly higher expression of p21 WAF1 and the lower expression of Rb were observed, whereas the expression of p53, p21 ras and C myc were not detected in DADS treated MGC803 cells. Effect of DMSO has similar effects as DADS.Conclusion DADS could induce an arrest in G 2 phase on human gastric cancer MGC803 cells partially, which could be involved in up regulation of p21 WAF1 and Rb gene expression and in down regulation of p53, ras and C myc gene expression.