胃泌素及其受体拮抗剂对原代培养胃癌细胞的作用研究

A Study of Gastrin and Its Receptor Antagonist on Primarily Cultured Gastric Cancer Cells

目的研究胃泌素及其受体拮抗剂对原代培养胃癌细胞的作用,探讨胃癌患者非细胞毒内分泌治疗的可行性。方法从新鲜胃癌切除标本中分离胃癌细胞进行体外培养,应用MTT比色分析法和流式细胞仪观察胃泌素及其受体拮抗剂丙谷胺对细胞增殖、周期分布的影响。结果胃泌素可明显促进部分胃癌细胞的增殖,使细胞从G0/G1期向S期和G2/M期转化,与对照组比较有显著差异(P<0.01),丙谷胺可阻断胃泌素的作用。对胃泌素敏感的胃癌原发部位多在胃体部(13/34)和贲门部(15/34),胃窦部较少(6/34);以低分化或未分化腺癌为主(77.1%),多为中晚期。结论胃泌素可促进部分胃癌患者肿瘤细胞的增殖和细胞内DNA合成,以胃体、贲门癌为著,胃泌素受体拮抗剂可望成为胃癌非细胞毒内分泌治疗新途径。

Objective To investigate the effects of gastrin and its receptor antagonist on primarily cultured gastric cancer cells, and to research the possibility of non cytotoxic endocrinous treatment for stomach cancer.Methods We observed the vitality and cycle distribution of gastric cancer cells under gastrin and proglumide in 34 cases, by MTT colorimetric assay and flow cytometric analysis.Results Gastrin could promote the proliferation of tumor cells in partial cases ( P < 0.01 ), and shifted G 0/G 1 phase to S and G 2/M phase( P < 0.05 )which could be blocked completely by proglumide; Gastric cancer which was susceptible to gastrin located in stomach body(13/34)and cardia(15/34) mostly, minoritly in pylorus(6/34), and with low or poor differentiation( 77.1% ), but being middle or late phase.Conclusion Gastrin can promote proliferation of partial gastric cancer cells and DNA synthesis; Gastrin receptor antagonist may become a non cytotoxic endocrinous treatment approach of some gastric cancer.