茶多酚对石英粉尘和烟溶液染毒大鼠肺脾锌、铜、镁含量的影响

Effect of tea polyphenols on the levels of Zn, Cu and Mg in lung and spleen in rats exposed to cigarettes smoking solution and silicon solution

目的 :探讨茶多酚 (TP)对石英粉尘 (SiO2 )与烟溶液 (CSS)单独及联合染毒大鼠肺及脾脏锌、铜、镁含量的影响。方法 :将 5 6只雄性Wistar大鼠随机分为 7组 ,正常对照组、SiO2 组、CSS组、SiO2 +CSS组和TP1、TP2、TP3处理组。除对照组外 ,各组采用非暴露式气管注入法染毒 ,TP1、TP2、TP3处理组于SiO2 及CSS联合染毒后分别给予 1.5 0g/ (kg·d)、2 .2 5g/ (kg·d)、3.30g/ (kg·d)的TP处理。实验结束动物称重后麻醉处死 ,取肺、脾称重并分别用原子吸收分光光度法测定其锌、铜、镁含量。结果 :与对照组比较 ,SiO2 组、CSS组及联合组大鼠脾、肺脏锌、镁含量下降 ,铜含量上升 (P <0 .0 5 ) ,且联合组与SiO2 组、CSS组比较 ,变化更显著 (P <0 .0 5 )。TP各处理组动物脾脏、肺脏锌、铜、镁含量均恢复到正常水平 ,与对照组比较差异无统计学意义 (P >0 .0 5 )。结论 :SiO2 与CSS单独作用时 ,均能使脾脏和肺脏受到损伤 ,而 2者联合作用时有协同作用 ;TP可拮抗SiO2 与CSS所致脾脏。

Aim: To study the effect of tea polyphenols (TP) on the levels of Zn, Cu and Mg in lung and spleen in rats exposed to cigaretts smoking solution and silicon solution. Methods: A total of 56 Wistar rats were divided into control group, SiO 2 group,CSS group, SiO 2+CSS group, the TP1, TP2 and TP3 treatment groups. The content of Zn, Cu, and Mg in lung and spleen were detected by the methods of AAS.Results: After the rats were exposed to smoking and silicon solution, the content of Zn and Mg in lung and spleen tissue significantly decreased, and that of Cu increased(P<0.05). However, in three TP treatment groups the content of Zn, Cu, and Mg in lung and spleen did not from those of the control(P>0.05). Conclusion: Tea polyphenols can prevent lung and spleen form ingurying induced by SiO 2 and CSS.