口腔鳞状细胞癌组织中Bax和CDK_4的表达

Expressions of Bax and CDK_4 in oral squamous cell carcinoma

目的 :研究Bax和CDK4蛋白在不同分级的口腔鳞状细胞癌组织中的表达 ,及其在口腔鳞癌发生发展过程中的作用及临床意义。方法 :利用免疫组织化学技术 ,检测Bax和CDK4蛋白在 10例口腔正常黏膜和 30例口腔鳞状细胞癌 (Ⅰ级 10例 ,Ⅱ级 10例 ,Ⅲ级 10例 )组织中的表达。结果 :在口腔鳞状细胞癌Ⅰ、Ⅱ、Ⅲ级组织中Bax蛋白阳性表达率分别为 90 .0 0 % (9/ 10 )、2 0 .0 0 % (2 / 10 )和 10 .0 0 % (1/ 10 ) ,其在口腔鳞状细胞癌组织中阳性表达强度呈递减的趋势 ,差异有统计学意义 (H =2 4 .78,P <0 .0 5 ) ;在口腔正常黏膜、口腔鳞状细胞癌组织中CDK4蛋白阳性表达率分别为 6 0 .0 0 % (6 / 10 )和 93.33% (2 8/ 30 ) ,差异有统计学意义 (H =6 .36 ,P <0 .0 5 ) ;在鳞癌Ⅰ、Ⅱ、Ⅲ级组织中 ,阳性表达率分别为 90 .0 0 % (9/ 10 )、90 .0 0 % (9/ 10 )和 10 0 % (10 / 10 ) ,3组之间差异无统计学意义 (P >0 .0 5 ) ;Bax与CDK4在口腔鳞状细胞癌组织中的表达呈明显的负相关关系 (r=- 0 .6 0 0 0 ,P <0 .0 5 )。结论 :Bax蛋白在口腔鳞癌表达的减少提示恶性程度增高预后较差 ;

Aim: To investigate the expression of Bax and CDK 4 in the normal oral mucosa and oral squamous cell carcinoma (OSCC). Methods: Bax and CDK 4 of 30 cases of OSCC(10 cases of GradeⅠ,10 cases of GradeⅡ, and 10 cases of GradeⅢ) and 10 cases of normal oral mucosa tissues were examined by immunohistochemical SABC technique. Results: The positive expression rates of Bax in GradeⅠ,Ⅱ, and Ⅲof OSCC were 90.00%(9/10),20.00%(2/10),and 10.00%(1/10), respectively,which decreased gradually. There was a significant difference among these 3 groups (H=24.78,P<0.05). The positive expression rates of CDK 4 in normal oral mucosa and OSCC were 60.00%(6/10) and 93.33%(28/30).There was a significant difference between the normal oral mucosa and OSCC (H=6.36,P<0.05), but there was no significant difference among the 3 groups of OSCC. There was a negative relationship between Bax and CDK 4 expressions in OSCC (r=-0.600 0, P<0.05). Conclusion: The decreasing of Bax protein and increasing of proliferating index of CDK 4 have a close relationship in carcinogenesis of OSCC. Bax protein can be a prognosis indicator of OSCC and its expression negatively correlates with the expression of CDK 4 in OSCC.