鼻用皮质类固醇对鼻息肉中白细胞介素5mRNA表达的影响

Effect of intranasal glucosteroid on the gene expression of interleukin-5 in nasal polyps

目的 探讨短期 (6～ 8周 )应用鼻用皮质类固醇治疗对鼻息肉组织中白细胞介素 5(interleukin 5 ,IL 5 )mRNA表达的影响。方法 采用合成地高辛标记的互补RNA探针进行原位杂交 ,观察经鼻用皮质类固醇———布地奈德治疗 6～ 8周和未经治疗的鼻息肉组织 (各 2 0例 )中 ,IL 5mRNA阳性细胞浸润的变化情况。结果 ①鼻息肉组织中IL 5mRNA阳性细胞主要为淋巴细胞和嗜酸粒细胞 ,且变应性 [(12 6± 4 6 ) / 0 2 5mm2 ]和非变应性患者 [(14 3± 4 1) / 0 2 5mm2 ]IL 5mRNA阳性细胞的密度差异无显著性 (t=- 0 775 ,P >0 0 5 ) ;②经鼻用皮质类固醇治疗的鼻息肉组织 [(10 2± 3 1) / 0 2 5mm2 ]的IL 5mRNA阳性细胞密度明显低于对照观察的鼻息肉组织 [(13 9±4 2 ) / 0 2 5mm2 ](t=3 114 ,P <0 0 1)。结论 短期 (6～ 8周 )应用鼻用皮质类固醇治疗可抑制鼻息肉组织中IL 5mRNA的表达水平。

Objective To investigate the effect of intranasal glucosteroid treatment on the expression of interleukin (IL)-5 gene in nasal polyps. Methods Nasal polyps from topical steroid treated patients (n=20) and untreated pati ents (n= 20) were investigated with the technique of mRNA in situ hybridizat ion. Results The majority of IL-5 mRNA positive cells in nasal polyps were lymphocytes or eo sinophils. No statistical significance was found in the densities of IL-5 mRNA positive cells between allergic patients [(12.6±4.6)/0.25mm2] and nona llergic patients [(14.3±4.1)/0.25mm2] (t=-0.775,P>0.05). Com pared with the control group [(13.9±4.2)/0.25mm2], the density of IL-5 mRNA positive cells was decreased in the steroid-treated group [(10.2±3.1)/ 0.25mm2], and the difference reached statistical significance (t=3.114 ,P<0.01). Conclusions These findings indicate that topical steroid treatment may suppress the IL-5 gene expression, and steroids may inhibit eosin ophil functions.