变应性鼻炎患者鼻腔灌洗液中细胞间黏附分子-1和白细胞介素-6水平及其与气道反应性的关系

Relationship between levels of intercellular adhesion molecule-1, interleukin-6 and airway hyperresponsiveness in patients with allergic rhinitis

目的 探讨变应性鼻炎患者鼻腔灌洗液中细胞间黏附分子-1(intercellular adhesionmolecule-1,ICAM-1)和白细胞介素-6(interleukin-6,IL-6)与气道反应性的关系。方法 采用双抗体夹心酶联免疫吸附测定法(enzyme-linked immunosorbent assay,ELISA)检测54例变应性鼻炎患者和20例健康对照,在特异性变应原鼻激发试验(specific allergen nasal provocation test,SANPT)后1h的鼻腔灌洗液中ICAM-1和IL-6的浓度。对54例变应性鼻炎患者和36例鼻炎合并支气管哮喘(缓解期)患者及20例健康对照进行肺功能检查和非特异性乙酰甲胆碱支气管激发试验,并采用直线相关分析方法分析其鼻腔灌洗液中ICAM-1、IL-6水平与肺功能第1秒用力呼气率(FEV1)的相关性。结果 变应性鼻炎患者鼻腔灌洗液中ICAM-1、IL-6的浓度分别为(272.75±32.25)pg/ml、(52.11±16.54)pg/ml,均明显高于健康对照组的(158.82±32.88)pg/ml和(25.64±10.14)pg/ml,差异有极显著意义(P均<0.01)。变应性鼻炎患者肺功能FEV1为(83.90±4.87)％,鼻炎合并哮喘患者为(78.82±7.41)％,健康对照为(90.25±4.69)％,三组间差异均有极显著意义(P均<0.01)。变应性鼻炎患者支气管激发试验阳性率为64.8％,鼻炎合并哮喘患者为83.33％,健康对照组无阳性,三组间差异均有极显著意义(

Objective To investigate the relation between levels of intercellular adhesion molecule-1, interleukin-6 and airway hyperresponsiveness in patients with allergic rhinitis. Methods Fifty-four patients with allergic rhinitis (AR) and 20 controls were included in the study. The levels of intercellular adhesion molecule-1 (ICAM-1 ) and interleukin-6 (IL-6) in nasal lavage fluid, gathered 1 hour after specific allergen nasal provocation test (SANPT) , were detected by sandwich enzyme-linked immunosorbent assay (ELISA) technique. The pulmonary function (FEVl) and nonspecific bronchial provocation test were measured in 54 patients with AR, 36 patients with AR and bronchial asthma (BA) and 20 controls. At the same time, the correlation between levels of ICAM-1 and IL-6 in nasal lavage fluid and pulmonary function (FEV1) was studied. Results The levels of ICAM-1 and IL-6 in nasal lavage fluid from patients with AR were (272.75±32. 25)pg/ml and(52. 11±16. 54)pg/ml, significantly higher than those the controls, which were (158. 82±33. 88) pg/ml and ( 25. 64±10. 14) pg/ml ( P < 0. 01). The pulmonary function (FEV1) in patients with AR and BA was (78. 82 ±7. 41) %. It was obviously lower than that in patients with AR [ (83. 90±4. 87 ) % ] , much lower than that in normal controls [ (90. 25 ±4. 69) % ] . The difference among them was significant. In patients with AR, the positive percentage of bronchial provocation test was 64. 81% , in patients with AR and BA, it was 83. 33% in normal controls, it was 0. The differences among them had very significant meaning. The levels of ICAM-1 and IL-6 in nasal provocation fluid had closely negative correlation with pulmonary function ( FEV1 ) , r = - 0. 7071, - 0. 6248, P < 0. 01. Conclusions The close correlation was observed in upper and lower airway for allergic inflammation. The pulmonary function of patients with AR was lower, and 64. 8% of them had airway hyperresponsiveness, so that they had the potent possibility to have bronchial asthma.