膀胱移行细胞癌组织中环氧化酶-2的表达及其意义

The significance of the expression of cyclooxygenase-2 in bladder transitional cell carcinoma tissues

目的 探讨环氧化酶 2 (COX 2 )在膀胱移行细胞癌组织中的表达及其临床意义。方法应用逆转录 聚合酶链反应 (RT PCR)检测 5 2例膀胱移行细胞癌患者的癌组织及 17例患者距离肿瘤组织 3cm以外的正常膀胱黏膜组织中COX 2mRNA的表达 ,应用Westernblot法检测其中 4 9例膀胱癌组织及全部 17例正常膀胱黏膜组织中COX 2蛋白的表达 ,并应用条带密度分析软件半定量分析Westernblot条带密度。结果 COX 2mRNA在 83% (43/ 5 2 )的癌组织中表达 ,仅在 2 9% (5 / 17)的正常膀胱黏膜组织中表达 (P <0 .0 0 1) ;4 9例膀胱癌组织COX 2蛋白的表达量与肿瘤的分级、分期相关 (P<0 .0 0 1)。结论 COX 2在膀胱移行细胞癌组织中高表达 ,可能在肿瘤的发生发展中起一定的作用 ,可能成为膀胱癌化学预防的一个靶点。

Objective To investigate the expression of cyclooxygenase 2 (COX 2) in bladder transitional cell carcinoma tissues, and understand its clinical significance. Methods Reversal transcription polymerase chain reaction (RT PCR) was used to assess the expression of COX 2 mRNA in 52 cases of bladder transitional cell carcinoma tissues and 17 cases of normal bladder tissues far from neoplasm; Western blot was used to assess the expression of COX 2 protein in 49 cases of bladder cancerous tissues and 17 cases of normal tissues. Results Positive expression of COX 2 mRNA was detected in 83%(43/52) of bladder cancer tissues and in 29%(5/17) of normal tissues by RT PCR and there was significant difference in expression of COX 2 mRNA between cancer tissues and normal tissues. Western blot analysis showed that expression of COX 2 protein was correlation with the stage and grade of cancer. Conclusions COX 2 is overexpressed in bladder transitional cell carcinoma. COX 2 maybe play a certain role in carcinogenesis and progression of bladder cancer and turn into a useful target of chemoprevention of bladder cancer.