期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

转基因植物检测体系在环境污染物突变性评价中的应用 被引量：1

The Application of Transgenic Plant in Evaluating the Genotoxicity of Environmental Contaminants
下载PDF
导出
摘要 很多环境污染物都能对生物体产生诱变性,可利用多种测试材料与方法对其基因毒性进行检测。转基因植物检测体系不仅能对环境污染的诱变性大小进行评价,而且还可以进一步分析其突变的分子机理。介绍了转基因植株检测体系的应用前景。 Environmental contaminants are powerful mutagenic factors for organisms. Several testing materials and methods have been used to assess the genotoxicity of environmental factors. Transgenic plants testing system can not only evaluate the level of genotoxicity, but also provide information on the genetic effects at molecular level. We introduce the use of transgenic plants in biomonitoring environmental factors.
作者 陈东 韩凝 马依群 朱睦元
机构地区 浙江大学生命科学学院 杭州市余杭区环境检测站
出处 《遗传》 CAS CSCD 北大核心 2004年第5期782-786,共5页 Hereditas（Beijing)
基金 国家自然科学基金项目资助(No.30370876 39770420)~~
关键词 环境检测 转基因工程 GUS基因 基因毒性 biomonitoring transgenic engineering GUS gene genotoxicity
分类号 Q789 [生物学—分子生物学]
  • 相关文献

参考文献18

  • 1Knasmuller S, Gottmann E, Steinkellner H. Detection of genotoxic effects of heavy metal contaminated soils with plant bioassays.Mutat Res, 1998, 420: 37-48.
  • 2Hartwig, A. Current aspects in metal genotoxicity. Biometals ,1995,8:3-11.
  • 3Rossam T G, Molina M, Boone P. Performance of 133 compounds in the lambda prophage induction endpoint of the Microscreen assay and a comparison with S. typhimurium mutagenicity and rodent carcinogenicity assays. Mutat Res, 1991 ,260:349～367.
  • 4Schaaper R M, Dunn R L. Spontaneous mutation in the Escherichia coli lcaI gene.Genetics , 1991,129:317-326.
  • 5Quillardet P, Touati E, Hofnung M. Contribution of the SOS chromotest to genetic toxicology.Mutat Res, 1997,379:s203.
  • 6Steinkellner H. Genotoxic effects of heavy metals: comparative investigation with plant bioassays. Environ Mol Mutagen ,1998,31:183-191.
  • 7Kong M S, Ma T H. Genotoxicity of contaminated soil and shallow well water detected by plant bioassays.Mut Res, 1999, 426:221-228.
  • 8曹佳.微核试验在中国的应用、发展与展望[J].遗传,2003,25(1):73-76. 被引量：63
  • 9Lohman P H,Vijg J; Uitterlinden A G,Slagboom P,Gossen J A,Berends F. DNA methods for detecting and analyzing mutations in vivo.Mutat Res, 1987,181:227-34.
  • 10Amanuma K, Takeda H, Amanuma H. Transgenic zebrafish for detecting mutations caused by compounds in aquatic environments.Nat Biotechnol , 2000, 18:62-65.

二级参考文献17

共引文献63

同被引文献101

  • 1Macek T, Sura M, Pavlikova D, Francova K, Scouten WH, Szekeres M, Sylvestre M, Mackova M. Can tobacco have a potentially beneficial effect to our health? Z Naturforsch C, 2005, 60(3/4): 292-299.
  • 2Novakova M, Mackova M, Chrastilova Z, Viktorova J, Szekeres M, Demnerova K, Macek T. Cloning the bacte- rial bphC gene into Nicotiana tabacum to improve the efficiency of PCB phytoremediation. Biotechnol Bioeng, 2009, 102(1): 29-37.
  • 3Gilbert ES, Crowley DE. Plant compounds that induce polychlorinated biphenyl biodegradation by Arthrobacter sp. strain BIB. ,4ppl Environ Microbiol, 1997, 63(5): 1933-1938.
  • 4Suenaga H, Watanabe T, Sato M, Ngadiman, Furukawa K. Alteration of regiospecificity in biphenyl dioxygenase by active-site engineering. J Bacteriol, 2002, 184(13): 3682-3688.
  • 5Barriault D, Plante MM, Sylvestre M. Family shuffling of a targeted bphA region to engineer biphenyl dioxygenase. JBacteriol, 2002, 184(14): 3794-3800.
  • 6Barriault D, Sylvestre M. Evolution of the biphenyl di- oxygenase BphA from Burkholderia xenovorans LB400 by random mutagenesis of multiple sites in region Ⅲ. J Biol Chem, 2004, 279(46): 47480-47488.
  • 7Zielinski M, Kahl S, Standfu [~-Gabisch C, C 6 mara B, Seeger M, Hofer B. Generation of novel-substrate-ac- cepting biphenyl dioxygenases through segmental random mutagenesis and identification of residues involved in enzyme specificity. Appl Environ Microbiol, 2006, 72(3): 2191-2199.
  • 8V 6 zina J, Barriault D, Sylvestre M. Family shuffling of soil DNA to change the regiospecificity of Burkholderia xenovorans LB400 biphenyl dioxygenase. J Bacteriol, 2007, 189(3): 779-788.
  • 9Fortin PD, Lo ATF, Haro MA, Kasehabek SR, Reineke W, Eltis LD. Evolutionarily divergent extradiol dioxygenases possess higher specificities for polychlorinated biphenyl metabolites. JBacteriol, 2005, 187(2): 415-421.
  • 10Susarla S, Medina VF, McCutcheon SC. Phytoremediation: an ecological solution to organic chemical contamination. Ecol Eng, 2002, 18(5): 647-658.

引证文献1

二级引证文献8

遗传
2004年 第5期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部