小鼠MⅡ卵母细胞中人类21号染色体同源基因的表达分析

The expression profiles of human chromosome 21 orthologues in mouse MⅡ oocyte

目的 考察人类 2 1号染色体 (HC2 1)同源基因在成熟的小鼠MⅡ卵母细胞中的表达模式 ,探讨其与早期胚胎发育以及唐氏综合征 (DS)表型发生的关系。方法 采用单个卵母细胞cDNAArray和GlobalRT PCR方法 ,对 93个人鼠同源的HC2 1基因在卵母细胞中的表达进行分析和鉴定。结果 93个基因中 ,有 2 6个在成熟的MⅡ卵母细胞成熟表达 ,这些基因涉及转录调节、代谢、离子通道和泛素作用等多个生物学环节。结论 表达模式的考察和验证表明这 2 6个基因直接参与正常胚胎发育的早期过程 ,这种表达模式的建立不仅为系统研究染色体不分离所致的基因表达失衡奠定了坚实的理论基础 。

Object To analyse the expression profile of orthologues of human chromosome 21 (HC21) in mouse MⅡoocytes, and to discuss the relationship between this expression profile and early embryonic development and further to find the possible reasons of DS phenotypes genesis Methods cDNA array and Global RT PCR methods were used to analyse and identify the expression profile of 93 HC21 orthologues in mouse MⅡoocytes Results 26 of 93 orthologues were proved to be expressed in mouse MⅡoocytes and these genes were involved in many biological procedure including transcriptional, metabolisim, ionic chunnel, and ubiquitin pathway etc Conclusion To our knowledge, this is first report about the HC21 orthologues expression profile in MⅡoocytes and this expression profile indicates that 26 of 93 HC21 orthologues may directly play important role in early development, and the biological information obtained from this experiment would be beneficial to understand imbalance of HC21 genes expression and the molecular mechanism of DS phenotypes genesis