银杏内酯对原代培养神经元缺氧诱导因子-1α基因表达的影响

EFFECT OF GINKGOLIDES ON GENE EXPRESSION OF HIF-1α IN PRIMARY CULTURED NEURONS

目的研究银杏内酯(ginkgolides,Gin)对单纯缺氧和模拟缺血神经元的缺氧诱导因子1α(hypoxiainduciblefactor1α,HIF1α)表达的影响。方法用RTPCR法检测原代培养胚胎小鼠皮层神经元经缺氧或剥夺氧和葡萄糖两种损伤处理,以及给予Gin(终浓度为37.5μg/ml)预保护时,HIF1αmRNA表达的变化。结果在培养的皮层神经元中HIF1αmRNA有一定的基础表达,Gin处理24h可明显提高HIF1α的表达水平;经单纯缺氧1h后,HIF1αmRNA表达上调,而预先给予Gin其表达强度进一步提高;但经剥夺氧和葡萄糖处理的神经元,HIF1αmRNA表达较未缺氧对照组明显降低,而预加Gin则能减轻HIF1αmRNA表达的下降。结论Gin对缺氧/缺血损伤神经元的HIF1αmRNA表达有上调作用。

Aim: To study the effects of ginkgolides(Gin) on the expression of hypoxia inducible factor 1(HIF 1α) in hypoxic/ischemic neurons. Methods: The gene expression of HIF 1α pretreated with or without Gin (37.5 μg/ml) was observed by RT PCR on primary cultured cortical neurons in the condition of hypoxia and oxygen glucose deprivation. Results: Some basic expresssion of HIF 1α mRNA were observed in cultured cortical neurons. The expression of HIF 1α mRNA increased after 24 h treatment with Gin. The level of HIF 1α mRNA increased also after 1 h hypoxia and further enhanced after the pretreatment with Gin. The expression of HIF 1α mRNA decreased with the deprivation of both oxygen and glucose, which reversed after the pretreatment of Gin. Conclusion: Gin could increase the expression of HIF 1α mRNA in hypoxic/ischemic cortical neurons.