顺铂诱导肾损伤过程中肾皮质脂质过氧化的变化

CHANGES OF RENAL CORTEX LIPID PEROXIDATION IN RENAL INJURY INDUCED BY CISPLATIN IN RATS

目的探讨顺铂肾损伤过程中肾皮质脂质过氧化与肾小管结构改变的关系。方法雌性Wistar大鼠随机分为生理盐水组、顺铂Ⅰ组、顺铂Ⅱ组、顺铂Ⅲ组,均为尾静脉注射给药,每天一次,连续五天。第六天取血测肌酐(Scr)、尿素氮(BUN)含量,取肾皮质测丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性、谷胱甘肽过氧化物酶(GSHPx)活性,同时进行肾小管上皮细胞碱性磷酸酶组织化学染色和组织病理学观察。结果顺铂组Scr、BUN明显升高,肾皮质MDA含量升高,SOD与GSHPx活性降低,与对照组相比均有显著差异(P<0.05),且肾皮质SOD活性、GSHPx活性与Scr、BUN含量呈明显负相关(P<0.05),肾皮质MDA含量与Scr、BUN含量呈明显正相关(P<0.05)。酶组化显示肾小管上皮细胞碱性磷酸酶大量丢失,病理切片结果显示肾皮质部分肾小管上皮细胞变性、坏死。结论顺铂引起肾皮质组织的破坏与肾皮质脂质过氧化增强有关,且随剂量增加肾皮质损伤加重。

Aim: To study the relativity of the renocortical lipid peroxidation with renal tubules structure damage in renal injury induced by cisplatin in rats.Methods: Female Wistar rats were randomly divided into NS group,CDDP Ⅰ group,CDDP Ⅱ group and CDDP Ⅲ group.All rats were injected via the tail vein with NS or cisplatin and NS qd in five days.The changes in content of Scr,BUN and MDA,the activity of SOD and GSH Px of the renal cortex were measured.Alkaline phosphatase of renal tubular epithelia was stained by histochemistry and the slices of renal cortex were observed.Results: The contents of Scr and BUN of CDDP groups were significantly higher than those of NS group( P <0.01).The content of renocortical MDA was significantly higher than that of NS group( P <0.05).The activities of renoconical SOD and GSH Px were lower than those of NS group( P <0.05).The content of MDA,activities of renocortical SOD and GSH Px with the content of Scr and BUN were significantly correlative.Alkaline phosphatase of renal tubular epithelia cells was losed largely and renal tubular epithelia cells were denaturative and necrotic partly in sections.Conclusion: The damage of renal cortex was correlative with its lipid peroxidation.The injury of renal cortex became heavier with cisplatin dose increased.