壮肝逐瘀煎抗大鼠肝纤维化的实验研究

<title>perimental study of anti-liver fibrosis of Zhuanggan Zhuyu decoction

[目的]探讨壮肝逐瘀煎抗肝纤维化的作用及其机制。[方法]采用CCl4复合因素大鼠肝纤维化模型,予壮肝逐瘀煎灌胃,观察血清学、形态学及免疫组织化学变化,并进行图像分析及统计学处理。[结果]壮肝逐瘀煎能显著降低大鼠血清透明质酸、层黏连蛋白、Ⅳ型胶原、Ⅲ型前胶原的水平;减轻肝脏炎症及纤维化程度:肝组织胶原面积明显减少;抑制肝组织α-平滑肌肌动蛋白和结蛋白的表达。[结论]壮肝逐瘀煎具有保肝和抗肝纤维化作用,其作用机制可能与抗炎、抑制肝脏星状细胞的活化和增殖有关。

<abstract>bjective] To investigate the effect and mechanism of Zhuanggan Zhuyu decoction (ZZD) in the treatment of liver fibrosis in rat model. [ Methods]The rat model of liver fibrosis was made by Carbon tetrachloride and combined factors and treated with ZZD. The indexes of hepatofibrosis, morphologic and immunohistochemical staining changes of the liver were observed. Image and statistical analysis were used to testify the results. [Results] After the treatment with ZZD, serum levels of HA, LN, Ⅳ-C and PC Ⅲ were significantly decreased; formation of liver fibrosis was successfully ameliorated; collagen staining positive area was decreased;and expression of α-SMA and HSC was inhibited. [Conclusion] ZZD could protect hepatocyte from injury and prevent the progress of hepatic fibrosis in rats, which may associate with its effect of anti - inflammation and inhibiting the activation and proliferation of HSC.