摘要
应用荧光薄层扫描法测定了化妆品中 L -抗坏血酸棕榈酸酯 (AP)的含量。选用高效硅胶 G薄层板 ,以三氯甲烷 -甲醇 -乙酸 -水 (体积比 :80∶ 2 0∶ 8∶ 2 )为展开剂 ,激发波长 λ=36 5 nm,AP在 1.0~ 16 .0 μg范围内呈良好的线性关系 ,回收率为 96 .4 %~ 10 5 .0 % ,RSD(相对标准偏差 ) =1.31% ,该法简单、灵敏、结果准确、重现性好 ,明显优于目前的其它各种分析方法 。
A thin layer chromatography fluorometry method was developed for the determination of ascorbyl palmitate(AP) in cosmetic. The silica gel G board and chloroform methanol acetic acid water (volume ratio:80∶20∶8∶2) as developer were chosen, λ =365 nm as scanning wavelength. The AP had a good linear relationship between 1.0 and 16.0 μg and the average recovery were 96.4%~105.0%,RSD=1.31%. The method is much better than the other of saponin determination and very satisfactory with simple, sensitive and the result is accurate with a good reproducibility. It can be also used for quality control of cosmetics.
出处
《武汉理工大学学报》
CAS
CSCD
2004年第11期39-41,49,共4页
Journal of Wuhan University of Technology
基金
湖南省自然科学基金 (0 3JJY5 0 0 1)
湖南省教育厅科研项目 (0 3C0 2 8)