高晶体-高胶体渗透压混合液对失血性休克大鼠不同组织中血管紧张素原表达的影响

Effects of hypertonic-hyperoncotic solution on the expression of angiotensinogen in different tissue in hemorrhagic shock rats

目的 研究高晶体 高胶体渗透压混合液 (HHS)复苏失血性休克时不同组织中血管紧张素原 (angiotensinogen ,ANG)表达的变化 ,探讨组织中的肾素 血管紧张素系统 (renin angiotensinsys tem ,RAS)活性的变化。方法 选取成年SD大鼠 30只 ,随机平均分为复方乳酸钠 (LRS)组和HHS组。制作失血性休克动物模型 ,分别用LRS或HHS复苏。采用逆转录酶 多聚酶链反应 (reverse tran scriptase polymerasechainreaction ,RT PCR)的方法半定量测定各组大鼠休克前、休克后和复苏后 30min的下丘脑、垂体前叶、肾上腺中的ANG基因表达水平 ,采用β actin为内对照。 结果失血性休克后各组织中ANG表达水平均明显升高。只有HHS可降低各组织中的升高的ANG的表达水平。结论 HHS可抑制失血性休克时组织中的RAS的激活。

Objective To study the effects of hypertonic-hyperoncotic solution(HHS) on the express of angiotensinogen(ANG)in different tissues in hemorrhagic shock rats. Methods Thirty adult SD rats were randomly divided into two equal groups: LRS (lactated Ringers’ solution) group and HHS group. Hemorrhagic shock animal model was built and rats were resuscitated with either LRS or HHS. Reverse transcriptase polymerase chain reaction (RT-PCR) was used to examine the relative abundance of ANG mRNA quantitatively in the hypothalamus, anterior pituitary, adrenal of rats of each group before shock, after shock, and at 30 min after resuscitation. Beta-actin was selected as a control. Results The expression of ANG in tissues were all significantly increased after hemorrhagic shock. The increases of expression were reduced only in HHS resuscitation group. Conclusion HHS can inhibit the activation of local RAS in hemorrhagic shock.