攻击性驾驶行为与单胺氧化酶A基因多态性的关系

Association between the functional monoamine oxidase A gene polymorphism and aggressively driving behavior

目的 探讨汽车驾驶员攻击性驾驶行为与单胺氧化酶A基因启动区可变数串联重复多态性的关系。方法 对 348例汽车驾驶员进行驾驶愤怒、驾驶报复、驾驶攻击问卷调查 ,根据量表得分 ,从中选择高、低攻击性驾驶员各 88人 ,采用聚合酶链反应 (PCR)和 3%琼脂糖电泳技术 ,检测MAOA基因启动区内含 30bp碱基的可变数串联重复多态性 ,并统计不同基因型在驾驶员中的分布。结果 发现 2种等位基因 ,分别是由 3个和 4个 30bp基因片段重复形成。这 2种等位基因在高、低攻击性驾驶员中的分布不均衡 ,与低攻击性驾驶员比 ,高攻击性驾驶员中含有 3个 30bp碱基的可变数串联重复多态基因型的构成比为 71 6 % (6 3/ 88) ,非常显著高于含有 4个 30bp碱基的可变数串联重复多态 (2 5 / 88) ,表明攻击性驾驶者含有较多的 3个 30bp碱基的可变数串联重复多态基因型。结论 攻击性驾驶行为可能与MAOA基因启动区可变数串联重复多态性有关 ,基因对攻击性驾驶行为的影响程度尚有待于进一步研究。

Objective This study is purposed to explore the relationship between aggressively driving behavior and functional polymorphism in the promoter region of the monoamine oxidase-A(MAOA)gene. Methods A total of 348 automobile drivers were investigated with Deffenbacher’s driver anger scale, driving vengeance questionnaire (DVQ) and driver aggression behavior questionnaire. Eighty-eight drivers were selected as more, medium and less aggressive group, each. Polymerase chain reaction (PCR) and 2.5% agarose gel electrophoresisi were adopted to detect the polymorphism of functional 30 bp-uVNTR in the promoter region of the X-chromosomal MAOA gene and their frequencies of varied genotypes were estimated. Results Two alleles with 3 and 4 repeats of 30 bp-uVNTR were detected in the drivers. Among the more aggressive group, number of the allele with 3 repeats of 30 bp-uVNTR (63/88) was significantly more than that with 4 repeats (25/88)(χ2=10.21, P<0.01), and number of the allele with 4 repeats of 30 bp-uVNTR was more in the less aggressive group, indicating that persons with allele of 3 repeats of 30 bp VNTR were more aggressive in their driving than those with 4 repeats. Conclusions Aggressively driving behavior in drivers possibly related to their functional MAOA-uVNTR polymorphism. Effect of the gene on aggressively driving behavior should be further studied.