阻垢剂——聚天冬氨酸的致突变性

Mutagenicity of Polyaspartic Acid

目的探讨阻垢剂——聚天冬氨酸的致突变性。方法选用昆明种小鼠,采用小鼠急性毒性实验(剂量设为20ml/kg)、Ames实验(剂量分别为5.000、1.000、0.200、0.040、0.008mg/皿)和小鼠骨髓嗜多染红细胞微核实验(剂量分别为5.00、2.50、1.25g/kg),研究了聚天冬氨酸的一般毒性和致突变性。结果采用霍恩法计算聚天冬氨酸小鼠经口半致死量(LD50)大于5000mg/kg;TA97、TA98、TA100和TA1024种菌株在+S9和-S9条件下的致突变率(MR)均小于2;小鼠骨髓嗜多染红细胞微核实验结果显示,阳性对照组与阴性对照组相比,微核率差异有显著性(P<0.01),而各剂量组雌、雄小鼠与阴性对照组相比,微核率差异无显著性(P>0.05)。结论聚天冬氨酸无毒,无致突变作用。

Objective To study the mutagenicity of polyaspartic acid (PASP), an incrustation inhibitor. Methods Acute toxicity test,Ames test and mouse marrow polychromatic erythrocyte micronucleus test were employed in the present paper. Results Oral LD50 for mice was greater than 5 000 mg/kg calculated by Horn method. The Ames test results showed that for TA97, TA98, TA100 and TA102, with or without S9, all MRs are <2. Mouse marrow polychromatic erythrocyte micronucleus test results were subject to Poisson distribution test (u-test), there was a significant difference had been seen between the positive control group and negative control group (P>0.01) and no significant difference between the PASP treated group and the negative control group (P>0.05). Conclusion PASP is an actually non-poisonous material and not a mutagen, the result of the present paper may be taken as a scientific evidence for the safety of PASP to be used in industry water treatment.