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共焦生物芯片扫描仪光束分离器设计探讨 被引量:1

Research on Design of Beamsplitter in Confocal Biochip Scanner
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摘要 指出激光共焦生物芯片扫描仪的光束分离器设计最终影响系统分辨率,简要给出了采用波长光束分离器和几何光束分离器的优缺点为满足仪器扫描分辨率和灵敏度这对互相受牵制的因素,系统地分析了几何光束分离器中,采用的反射镜孔径的定量分析计算方法所得的结果对几何光束分离器的设计和性能的评价有重要的意义。 The influence of the design of beamsplitter in confocal biochip scanner upon system′s resolution is pointed out, then the two designs adapting dichroic or multichroic beamsplitter and geometric beamsplitter are listed and compared with each other briefly. A quantitive calculative method of the hole diameter of reflector in geometric beamsplitter, according with the demand of the scanning resolution and sensitivity a pair of counteractive factors, is analysed mainly. The result is very useful for the design and characteristic evaluation of geometric deamsplitter, and the experiment results are given.
出处 《光子学报》 EI CAS CSCD 北大核心 2004年第11期1382-1385,共4页 Acta Photonica Sinica
基金 国家重点自然科学基金项目
关键词 共焦扫描 光束分离器 分辨率 灵敏度 Confocal scan Beamsplitter Resolution Sensitivity
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参考文献5

  • 1Fodor S P A, Rava R P, Huang X C,et al. Multiplexed biochemical assays with biological chips. Nature, 1993,364(6437): 555-556
  • 2吴岚君. 生物芯片(DNA微阵列)荧光扫描仪中的激光共聚焦扫描技术. 基因快讯, 2001,(3) Wu L J. Gene News, 2001,(3)
  • 3Gu M, Sheppard C J R.Effects of a finite-sized detector on the OTF of Confocal Fluorescent Microscopy.Optik, 1991,89(2) : 65-69
  • 4Wilson T,Carlini A R.Three-dimensional imaging in confocal imaging systems with finite sized detectors.J Microsc, 1988,149(1): 51-66
  • 5Full Moon Biosystems.Inc.. Microarray Scanner Calibration Slide User's Guide. Part Number: FMB DS 01, 2-9

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  • 5WILSON P K,JIANG T,MINUNNI M E,etal. A novel optical biosensor format for the detection of clinically relevant TP53 mutations [ J ]. Biosensors and Bioelectronics, 2005, 20 : 2310 -2313.
  • 6GOMMANS W M, HAISMA H J, ROTS M G. Engineering zinc finger protein transcription factors: The therapeutic relevance of switching endogenous gene expression on or off at command[J]. J Mol Biol,2005,354:507- 519.
  • 7CHOI J W,AHN W S,BAE S M,et al . Adenoviral p53 effects and cell-specific E7 protein-protein interactions of human cervical cancer cells [J ]. Biosensors and Bioelectronics, 2005, 20:2236- 2243.
  • 8DILLON P P, MANNING B M, DALY S J,et al. Production of recombinant anti-morphine-3-glucuronide single chain variable fragment (scFv) antibody for the development of a " real time" biosensor based immunoassay [ J ]. Journal of Immunological Methods ,2003,276:151 -161.
  • 9STEINER G, Surface plasmon resonance imaging [J], Anal Bioanal Chem, 2004,379 : 328-331.
  • 10王立强,陆祖康,倪旭翔,郑旭峰,李映笙.共聚焦生物芯片扫描仪中PMT电流增益的自动控制[J].光子学报,2004,33(3):314-317. 被引量:5

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