干扰素-γ对小鼠哮喘模型气道炎症和肺T淋巴细胞及血浆IgE的影响

Effect of interferon-gamma on airway inflammation and T lymphoid cells in lung and serum IgE level in murin asthmatic models

[目的]探讨干扰素-γ(IFN-γ)对支气管哮喘(哮喘)小鼠气道炎症及肺T淋巴细胞和血浆中IgE的影响。[方法]C57BL／6小鼠随机分为正常对照组(A组,10只)、哮喘模型组(B组,10只)、IFN-γ注射组(C组,10只)。采用卵蛋白(OVA)和氢氧化铝致敏、雾化建立哮喘模型,B组、C组分别在致敏同时第1、3、5、9、15、17、20 d,1次／d腹腔注射生理盐水(0.1 m1)和IFN-γ(1 500U)。第22 d收取肺泡灌洗液(BALF)并取其肺T淋巴细胞体外培养及血浆中IgE。分析小鼠BALF和肺部淋巴细胞产生的细胞因子IL-4、IL-5及血浆中IgE水平的变化。[结果]A组无症状,B组哮喘鼠症状重,C组哮喘鼠症状轻。在BALF中嗜酸性粒细胞百分比(EOS％)计数A组0,B组20.1±7.0,C组0.7±0.2。哮喘模型组肺T淋巴细胞中IL-4、IL-5,血浆中IgE显著高于正常对照组(P<0.01),治疗组肺T淋巴细胞中IL-4、IL-5,血浆中IgE显著低于哮喘模型组(P<0.01)。[结论]IFN-γ可以抑制哮喘小鼠气道炎症,其机制之一可能与是抑制肺T淋巴细胞产生的IL-4、IL-5,降低血清中总IgE水平有关。

[Objective] To investigate the effects of interferon-gamma (IFN-γ) on airway inflammation, T lymphoid cells in lung and serum IgE level in murin asthmatic models. [Methods] C57BL/6 mice were randomly divided into three group: group A (control group, n = 10); group B (asthmatic model group, n = 10); group C (IFN-γ injection group, n = 10). The asthmatic model in group B and C were established by ovalbumin (OVA) and aluminum hydroxide absorbtion. Saline (0.1 ml) and IFN-γ(1 500U) were respectively injected in group B and C on day 1、3、5、9、15、17、20. The cellulal composition of bronch-oalveolar lavage fluid (BALF) on day 22, T lymphoid cells in lung vitro and serum IgE were taken. The cellular composition of BALF, the cytokines secreted IL-4、IL-5 by lung T lymphoid cells and serum IgE level were analised. [Results] The symptoms of group B were more severe than that of group A and C. Group A did not have eosinophil (EOS) in the BALF, while the EOS% in group B and group C was 20.1±7 .0、0.7±0.2、IL-4、IL-5 production from lung T lymphoid cells and IgE level in serum were markedly increased of murin models groups with significant differences between the control healthy mice (P< 0.01). IL-4、IL-5 production from lung T lymphoid cells and IgE level in serum were markedly reduced in IFN-7 injection groups with significant differencesbetween the asthmatic model (P<0.0l). [Conclusion] IFN-γ could inhibit airway inflammtion in asthmatic mice. One of its mechamism may be that it can inhibitIL-4 and IL-5 prodution from lung T lymphoid cells and reduc IgE level in serum.