中枢神经系统损伤过程中Nogo-A降解片段的检测

DETECTION OF Nogo-A PROTEOLYTIC FRAGMENT DURING CNS INJURY

目的 观察大鼠脊髓横断、脑缺血和海人藻酸 (KA)注射所致的脑损伤模型Nogo A的降解情况 ,以推测Nogo A降解片段产生的机制和可能的作用。 方法 制作大鼠脊髓横断、脑缺血和KA注射所致的脑损伤模型 ,Western免疫印迹方法检测Nogo A降解片段。 结果 大鼠脊髓横断和脑缺血模型均检测到Nogo A降解片段 ,而KA注射所致的脑损伤模型未发现Nogo A降解片段。 结论 CNS机械性和缺血性损伤可能通过某些机制导致Nogo A发生降解。

Objective To detect Nogo-A proteolytic fragment in CNS injury models and investigate its proteolytic mechanism as well as potential functions. Methods Western blot was performed to detect Nogo-A proteolytic fragment after spinal cord transection,brain ischemia and KA-induced cerebral cortex injury in rats. Results We detected Nogo-A proteolytic fragment in spinal cord transection and brain ischemia models,whereas no Nogo-A proteolytic fragment was found in KA-induced cerebral cortex injury model.Conclusion\ CNS mechanical and ischemic injury may induce the degradation of Nogo-A by certain mechanisms.