体外培养脊髓神经元损伤后c-Jun蛋白表达

EXPRESSION OF c-Jun PROTEIN IN INJURED CULTURED SPINAL CORD NEURONS

目的 建立一个模拟脊髓全横断损伤后脊髓组分———原代培养的脊髓神经元损伤的模型。探讨中枢神经损伤后损伤应答基因c jun的表达及变化。 方法 取Wistar大鼠 (孕 14d)脊髓 ,培养 10～ 12d后 ,采用所设计的标准模板 ,直视下进行损伤。观察损伤前、后不同时间神经元的形态及即刻早期基因c jun的表达及变化。 结果 脊髓神经元损伤 10min后胞核即有c Jun标记 ,损伤 2h后最多 ,而且阳性神经元的密度与划痕之间的距离呈明显的反比关系。 结论 受机械损伤的神经元中 ,c Jun表达阳性意味着此即刻早期基因产物已进入细胞核内起着“第三信使”的作用。

Objective To establish a model of injury to primarily cultured spinal cord neurons,mimicking the neuronal injury after complete transactional spinal cord trauma,for the sake of exploring changes in expression of an immediately-early gene,c-jun,in central nervous system injury. Methods Spinal cords were removed form fetal Wistar rats at the 14th gestation day and the neurons were cultured for 10 to 12 days.Then,mechanical injury was applied to the neurons by making regular scores on the culture disk under direct vision with the aid of a self-made standard template.Morphology of the injured neurons and changes in expression of c-Jun protein were observed before and at different intervals after injury. Results c-Jun expression was noted in neuronal nuclei 10 min after injury and its peak appeared at 2 hrs.Besides,the density of positive neurons bore evidently an inverse proportion with their distance from the scores.Conclusion\ Positive expression in injury neurons show that c-jun gene enters the nucleoli of injured neurons and takes the role of “the third messenger” at early time after neuronal injury.