施万细胞和L-NNA对脊髓半横断后脑干红核神经元存活的影响

EFFECTS OF SCHWANN CELLS AND L-NNA ON THE NEURONALSURVIVAL IN RED NUCLEUS OF BRAIN STEM AFTERSPINAL CORD HEMISECTION IN RAT

目的 探讨移植施万细胞 (SCs)和应用一氧化氮合酶抑制剂L 硝基精氨酸 (L NNA)能否促进脑干红核受损伤的神经元存活。 方法 2 0只成年SD雌性大鼠分为 :对照组、SCs组、L NNA组和SCs +L NNA组。在胸11脊髓段半横断后立即在损伤处植入SCs,术后腹腔注射L NNA。 结果 脊髓半横断后 30d ,对照组和L NNA组大鼠受损伤侧红核的神经元密度明显降低 ,但两组间无明显差异 ;两组受损伤侧红核神经元的NOS表达阴性。SCs组和SCs +L NNA组大鼠受损伤侧红核的神经元密度明显增加 ,两组间无显著差异 ;两组受损伤侧红核神经元NOS表达也呈阴性。 结论 移植的SCs能够促进受损伤的红核神经元存活。NOS可能不参与影响受损伤红核神经元的存活过程 ,因而L NNA对受损伤红核神经元的存活没有作用。

Objective To explore whether combination of Schwann cells(SCs) and Nomega-nitro-L-arginine(L-NNA,inhibitor of nitric oxide synthase) could promote injured neuronal survival in red nucleus(RN) of brain stem. Methods Twenty adult female rats were divided into control group,SCs group,L-NNA group and SCs+L-NNA group.SCs were immediately implanted into the lesioned site after hemisection of T 11 spinal cord segment and L-NNA was intraperitoneally injected in SCs+L-NNA group. Results The neuronal densities of injured RN decreased obviously 30 days after spinal cord hemisection in control and L-NNA groups.However,there was no difference in the neuronal densities between two groups.The activity of NOS was not detected in injured RN neurons.In SCs and SCs+L-NNA groups,the neuronal densities of injured RN were obviously higher and there was no difference in the neuronal densities between two groups.The activity of NOS also was not detected in injured RN neurons.Conclusion\ SCs transplantation may promote the survival of injured RN neurons.NOS may take part in affecting the survival of injured RN neurons and L-NNA may not play a role in the survival of injured RN neurons.