氯化锂促进不同年龄成年小鼠齿状回细胞增殖与神经发生

LITHIUM CHLORIDE INCREASES CELL PROLIFERATION AND NEUROGENESIS IN THE ADULT MOUSE DENTATE GYRUS

目的 观察氯化锂 (LiCl)对不同年龄成年小鼠齿状回细胞增殖与神经发生的影响。 方法 以氯化锂喂养动物 ,用 5 溴 2′ 脱氧尿核苷 (BrdU)标记新生细胞 ,免疫组织化学染色后统计新生细胞数量 ;应用BrdU与TuJl/GFAP双标记 ,鉴别出BrdU阳性细胞的细胞类型。 结果 不同年龄成年小鼠齿状回的BrdU阳性细胞形态无明显差别 ,4 8周龄组阳性细胞的数量明显低于 8周龄组 ;LiCl组小鼠新增殖细胞明显多于对照组 ,其中约有 70 %为神经元 ,4 %～ 5 %左右为神经胶质细胞。 结论 服用LiCl可显著增强成年小鼠齿状回的细胞增殖与神经发生 ,部分逆转其随年龄增长而逐渐减低的趋势 ,对于中枢神经系统退行性疾病的预防与治疗可能有重要的意义。

Objective To study the effect of lithium chloride on cell proliferation and neurogenesis in the adult mouse dentate gyrus. Methods Mice were chronically treated with lithium chloricle for 4 weeks and proliferating cells were labeled with 5-bromo-2′-deoxyuridine (BrdU) over 10 days. BrdU immunohistochemistry was used to dertermine the number of newly-generated cells and double immunolabeling was undertaken to co-localize BrdU-positive cells with TuJ1/GFAP. Results There is no obviously difference in the morphology of BrdU-positive cells among the adult mice of different age. The number of BrdU-labeled cells in the 48 week group is much less than that of the 8 week group. Lithium chloride produced a significant 20% increase in the BrdU-labeled cells in the dentate gyrus, 70% of which were double-labeled with BrdU and TuJ1, 4%-5% with BrdU and GFAP.Conclusion These findings suggest that lithium chloride can significantly increase cell proliferation and neurogenesis in the adult mouse dentate gyrus and partly restore neurogenesis in aged mice, which imply that lithium may be useful in the treatment of degenerative CNS diseases.